2019 ; Vol.29-8: 1230~1239
|Author||Qiang Fu, Lu Gao, Xiao Fu, Qinghua Meng, Zhihong Lu|
|Place of duty||Department of cardiovascular surgery. The general hospital of Tianjin medical university, Tianjin, 300070, China|
|Title||Scutellaria baicalensis Inhibits Coxsackievirus B3-Induced Myocarditis Via AKT and p38 Pathways|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Scutellaria baicalensis Georgi has been widely used in China for treatment of various diseases.
This study investigated the effect of Scutellaria baicalensis Georgi extracts (SBE) against
Coxsackievirus B3 (CVB3)-induced myocarditis in vitro and in vivo. In vitro, Hela cells and
primary myocardial cells were infected with CVB3 and treated with SBE (50-800 μg/ml) and
ribavirin (200 μM) for 48 h and then determined by CCK8 assay. Real-time PCR and western
blotting assays were performed. In vivo, a myocarditis model was induced in male BALB/c
mice by injecting CVB3 suspension intraperitoneally for three times, followed by treatment
with SBE (400 and 200 mg/kg) and ribavirin (100 mg/kg) for 28 days. SBE ameliorated the
cytotoxicity of CVB3 in Hela cells, especially at 400 μg/ml (39.93% vs 65.67%, p < 0.05) without
influencing cell growth and also significantly reduced CVB3 replication in primary
myocardial cells. The levels of AKT, ERK, and p38 were increased after CVB3 infection. SBE
could downregulate the expressions of AKT and p38. In vivo, the mortality rate from CVB3
reached to 66.67%, while 10.00% and 23.33% of this came after 400 and 200 mg/kg SBE
treatment, respectively (p < 0.05). The CVB3 replication was obviously reduced after SBE
administration from day 5. Similarly, the levels of AKT, ERK, and p38 mRNAs and proteins
were increased, and SBE suppressed the expression of AKT and p38. Our study indicates that
SBE is a promising potent antiviral agent against CVB3-induced myocarditis by inhibition of
virus replication via depressing AKT and p38 expressions.|
|Key_word||Scutellaria baicalensis, Coxsackievirus B3, myocarditis, AKT, p38|
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