2019 ; Vol.29-8: 1288~1298
|Author||Xiaochen Wang, Hongyu Yang, Wei Zhou, Jun Liu, Ning Xu|
|Place of duty||University of Science and Technology of China, Hefei 230026, P.R. China|
|Title||Deletion of cg1360 Affects ATP Synthase Function and Enhances Production of L-Valine in Corynebacterium glutamicum|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Bacterial ATP synthases drive ATP synthesis by a rotary mechanism, and play a vital role in
physiology and cell metabolism. Corynebacterium glutamicum is well known as an industrial
workhorse for amino acid production, and its ATP synthase operon contains eight structural
genes and two adjacent genes, cg1360 and cg1361. So far, the physiological functions of Cg1360
(GenBank CAF19908) and Cg1361 (GenBank CAF19909) remain unclear. Here, we showed that
Cg1360 was a hydrophobic protein with four transmembrane helices (TMHs), while no TMH
was found in Cg1361. Deletion of cg1360, but not cg1361, led to significantly reduced cell
growth using glucose and acetic acid as carbon sources, reduced F1 portions in the membrane,
reduced ATP-driven proton-pumping activity and ATPase activity, suggesting that Cg1360
plays an important role in ATP synthase function. The intracellular ATP concentration in the
Δcg1360 mutant was decreased to 72% of the wild type, while the NADH and NADPH levels
in the Δcg1360 mutant were increased by 29% and 26%, respectively. However, the Δcg1361
mutant exhibited comparable intracellular ATP, NADH and NADPH levels with the wild-type
strain. Moreover, the effect of cg1360 deletion on L-valine production was examined in the
L-valine-producing V-10 strain. The final production of L-valine in the V-10-Δcg1360 mutant
reached 9.2 ± 0.3 g/l in shake flasks, which was 14% higher than that of the V-10 strain. Thus,
Cg1360 can be used as an effective engineering target by altering energy metabolism for the
enhancement of amino acid production in C. glutamicum.|
|Key_word||ATP synthase, cg1360, C. glutamicum, L-valine production|
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