2019 ; Vol.29-7: 1061~1070
|Author||Ju-Hee Min, Lebaka Veeranjaneya Reddy, Charalampopoulos Dimitris, Young-Min Kim, Young-Jung Wee|
|Place of duty||Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Republic of Korea|
|Title||Optimized Production of Poly(γ-Glutamic acid) By Bacillus sp. FBL-2 through Response Surface Methodology Using Central Composite Design|
J. Microbiol. Biotechnol.2019 ;
|Abstract||In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus
sp. FBL-2 was studied using a statistical approach. One-factor-at-a-time method was used to
investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was
utilized to select the most significant nutrients affecting the yield of γ-PGA. After identifying
effective nutrients, response surface methodology with central composite design (CCD) was
used to obtain a mathematical model to identify the optimum concentrations of the key
nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA
production. The optimum amount of significant medium components appeared to be sucrose
51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l. The
optimized medium was validated experimentally, and γ-PGA production increased
significantly from 3.59 g/l (0.33 g/l/h) to 44.04 g/l (3.67 g/l/h) when strain FBL-2 was
cultivated under the optimal medium developed by the statistical approach, as compared to
|Key_word||Poly(γ-glutamic acid), L-glutamic acid, Bacillus sp. FBL-2, optimization, response surface methodology|
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