2019 ; Vol.29-8: 1212~1220
|Author||Junsang Oh, Eunhyun Choi, Deok-Hyo Yoon, Tae-Yong Park, Bhushan Shrestha, Hyung-Kyoon Choi, Gi-Ho Sung|
|Place of duty||Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary’s Hospital and College of Medicine, Catholic Kwandong University, Incheon 22000, Republic of Korea ,College of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea|
|Title||1H-NMR-Based Metabolic Profiling of Cordyceps militaris to Correlate the Development Process and Anti-Cancer Effect|
J. Microbiol. Biotechnol.2019 ;
|Abstract||The study of metabolomics in natural products using the diverse analytical instruments
including GC-MS, LC-MS, and NMR is useful for the exploration of physiological and
biological effects and the investigation of drug discovery and health functional foods.
Cordyceps militaris has been very attractive to natural medicine as a traditional Chinese
medicine, due to its various bioactive properties including anti-cancer and anti-oxidant
effects. In this study, we analyzed the metabolite profile in 50% ethanol extracts of C. militaris
fruit bodies from three development periods (growth period, matured period, and aging
period) using 1H-NMR, and identified 44 metabolites, which are classified as 16 amino acids,
10 organic acids, 5 carbohydrates, 3 nucleotide derivatives, and 10 other compounds. Among
the three development periods of the C. militaris fruit body, the aging period showed
significantly higher levels of metabolites including cordycepin, mannitol (cordycepic acid),
and β-glucan. Interestingly, these bioactive metabolites are positively correlated with antitumor
growth effect; the extract of the aging period showed significant inhibition of HepG2
hepatic cancer cell proliferation. These results showed that the aging period during the
development of C. militaris fruit bodies was more highly enriched with bioactive metabolites
that are associated with cancer cell growth inhibition.|
|Key_word||Cordyceps militaris, metabolomics, cordycepin, β-glucan, nuclear magnetic resonance, anti-cancer effect|
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