2019 ; Vol.29-7: 1043~1052
|Author||Jing Zhu, Yanjing Liu, Yan Qin, Lixia Pan, Yi Li, Ge Liang, Qingyan Wang|
|Place of duty||State Key Laboratory of Non-Food Biomass Energy and Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Bioscience and Technology Research Center, Guangxi Academy of Sciences, Nanning 530007, China|
|Title||Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a
modified trypan blue and tetracycline, ampicillin (TB-TA) plate. The electro-phoretically pure
enzyme was obtained by purification using ethanol precipitation, ion-exchange
chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and
the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest
activity after hydrolysis with p-NPC16 at a pH of 8.5 and 50°C, and the Km, kcat, and kcat/Km
values were 1.05, 292.95 s-1 and 279 s-1mM-1, respectively. The lipase was highly stable at 7.5 ≤
pH ≤ 10.0. K+ and Na+ exerted activation effects on the lipase which had favorable tolerance
to short-chain alcohols with its residual enzyme activity being 110% after being maintained in
30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli
Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical
properties for a range of applications in the food-processing and detergent industries, and has
particularly high potential for use in the manufacture of biodiesel.|
|Key_word||Burkholderia gladioli, alkaline Lipase, purification, biocatalysis|
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