2019 ; Vol.29-7: 1096~1103
|Author||Mojde Moradipour, Roohallah Saberi-Riseh, Reza Mohammadinejad, Ahmad Hosseini|
|Place of duty||Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan 7718897111, Iran|
|Title||Nano-Encapsulation of Plant Growth-Promoting Rhizobacteria and Their Metabolites Using Alginate-Silica Nanoparticles and Carbon Nanotube Improves UCB1 Pistachio Micropropagation|
J. Microbiol. Biotechnol.2019 ;
|Abstract||UCB-1 is the commercial rootstock of pistachio. Reproduction of this rootstock by tissue
culture is limited by low levels of proliferation rate. Therefore, any compound that improves
the proliferation rate and the quality of the shoots can be used in the process of commercial
reproduction of this rootstock. Use of plant growth-promoting bacteria is one of the best ideas.
Given the beneficial effects of nanoparticles in enhancement of the growth in plant tissue
cultures, the aim of the present study was to investigate the effects of nanoencapsulation of
plant growth-promoting rhizobacteria (using silica nanoparticles and carbon nanotubes) and
their metabolites in improving UCB1 pistachio micropropagation. The experiment was
conducted in a completely randomized design with three replications. Before planting,
treatments on the DKW medium were added. The results showed that the use of Pseudomonas
fluorescens VUPF5 and Bacillus subtilis VRU1 nanocapsules significantly enhanced the root
length and proliferation. The nanoformulation of the VUPF5 metabolite led to the highest root
length (6.26 cm) and the largest shoot (3.34 cm). Inoculation of explants with the formulation
of the metabolites (both bacterial strains) significantly elevated the average shoot length and
the fresh weight of plant compared to the control. The explants were dried completely using
both bacterial strains directly and with capsule coating after the three days.|
|Key_word||Carbon nanotube, SiO2 nanoparticle, UCB1, PGPR, micropropagation|
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