2019 ; Vol.29-5: 721~730
|Author||YunJung Lee, Yu Ra Ji, Sumi Lee, Mi-Jung Choi, Youngjae Cho|
|Place of duty||Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Republic of Korea|
|Title||Microencapsulation of Probiotic Lactobacillus acidophilus KBL409 by Extrusion Technology to Enhance Survival under Simulated Intestinal and Freeze-Drying Conditions|
J. Microbiol. Biotechnol.2019 ;
|Abstract||The probiotic Lactobacillus acidophilus KBL409 was encapsulated with alginate (Al) and
alginate-chitosan (Al/Chi) through extrusion method. The sizes and zeta potentials of
microspheres were measured to confirm encapsulation. To evaluate the protective effect of
microspheres against gastrointestinal fluids, all the samples were exposed to simulated gastric
fluids (SGFs, pH 1.5) at 37°C for 1 or 2 h, followed by incubation with simulated intestinal
fluids (SIFs, pH 6.5) for 2 h. The mucoadhesive ability of microspheres was evaluated using
the intestinal epithelial cell line HT29-MTX. To extend the shelf-life of probiotics,
lyoprotectants such as disaccharide and polysaccharide were mixed with free or encapsulated
cells during the freeze-drying process. The size of the microspheres demonstrated a narrow
distribution, while the zeta potentials of Al and Al/Chi-microspheres were −17.9 ± 2.3 and
20.4 ± 2.6 mV, respectively. Among all the samples, Al/Chi-encapsulated cells showed the
highest survival rate even after exposure to SGF and SIF. The mucoadhesive abilities of Al and
Al/Chi-microspheres were higher than 94%, whereas the free L. acidophilus showed 88.1%
mucoadhesion. Ten percent of sucrose showed over 80% survival rate in free or encapsulated
cells. Therefore, L. acidophilus encapsulated with Al and Al/Chi-microspheres showed higher
survival rates after exposure to the gastrointestinal tract and better mucoadhesive abilities
than the free cells. Also, sucrose showed the highest protective effect of L. acidophilus during
the freeze-drying process.|
|Key_word||Encapsulation, extrusion, Lactobacillus acidophilus, intestinal survival, mucoadhesion, lycoprotectant|
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