2019 ; Vol.29-7: 1022~1032
|Author||Hyung-Seok Yu, Na-Kyoung Lee, Ae-Jin Choi, Jeong-Sook Choe, Chun Ho Bae, Hyun-Dong Paik|
|Place of duty||Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Republic of Korea|
|Title||Anti-Inflammatory Potential of Probiotic Strain Weissella cibaria JW15 Isolated from Kimchi through Regulation of NF-κB and MAPKs Pathways in LPS-Induced RAW 264.7 Cells|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Probiotics are known to provide the host with immune-modulatory effects and are therefore of
remarkable interest for therapeutic and prophylactic applications against various disorders,
including inflammatory diseases. Weissella cibaria JW15 (JW15) has been reported to possess
probiotic and antioxidant properties. However, the effect of JW15 on inflammatory responses
has not yet been reported. Therefore, the objective of the current study was to evaluate the
anti-inflammatory potential of JW15 against lipopolysaccharide (LPS) stimulation. The
production of pro-inflammatory factors and the cellular signaling pathways following
treatment with heat-killed JW15 was examined in LPS-induced RAW 264.7 cells. Treatment
with heat-killed JW15 decreased nitric oxide and prostaglandin E2 production via downregulation
of the inducible nitric oxide synthase and cyclooxygenase-2. In addition, treatment
with heat-killed JW15 suppressed the expression of pro-inflammatory cytokines, interleukin
(IL)-1β, IL-6, and tumor necrosis factor-α. The anti-inflammatory properties of treating with
heat-killed JW15 were associated with mitogen-activated protein kinase signaling pathwaymediated
suppression of nuclear factor-κB. These results indicated that JW15 possesses antiinflammatory
potential and provide a molecular basis regarding the development of
functional probiotic products.|
|Key_word||Weissella cibaria JW15, kimchi, probiotics, anti-inflammation, nuclear factor-κB, mitogen activated protein kinase|
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