2019 ; Vol.29-9: 1391~1400
|Author||Jun Seok Park, Robin B Guevarra, Bo-Ra Kim, Jun Hyung Lee, Sun Hee Lee, Jae Hyoung Cho, Hyeri Kim, Jin Ho Cho, Minho Song, Ju-Hoon Lee, Richard E Isaacson, Kun Ho Song, Hyeun Bum Kim|
|Place of duty||College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea|
|Title||Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Canine parvoviral enteritis (PVE) is an important intestinal disease of the puppies; however,
the potential impact of the canine parvovirus (CPV) on the gut microbiota has not been
investigated. Therefore, the aim of this study was to evaluate the gut microbial shifts in
puppies naturally infected with CPV. Fecal samples were collected from healthy dogs and
those diagnosed with PVE at 4, 6, 8, and 12 weeks of age. The distal gut microbiota of dogs was
characterized using Illumina MiSeq sequencing of the bacterial 16S rRNA genes. The sequence
data were analyzed using QIIME with an Operational Taxonomic Unit definition at a
similarity cutoff of 97%. Our results showed that the CPV was associated with significant
microbial dysbiosis of the intestinal microbiota. Alpha diversity and species richness and
evenness in dogs with PVE decreased compared to those of healthy dogs. At the phylum level,
the proportion of Proteobacteria was significantly enriched in dogs with PVE while
Bacteroidetes was significantly more abundant in healthy dogs (p < 0.05). In dogs with PVE,
Enterobacteriaceae was the most abundant bacterial family accounting for 36.44% of the total
bacterial population compared to only 0.21% in healthy puppies. The two most abundant
genera in healthy dogs were Prevotella and Lactobacillus and their abundance was significantly
higher compared to that of dogs with PVE (p < 0.05). These observations suggest that
disturbances of gut microbial communities were associated with PVE in young dogs.
Evaluation of the roles of these bacterial groups in the pathophysiology of PVE warrants
|Key_word||Gut microbiota, canine parvovirus, dogs, 16S rRNA|
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