Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-9: 1391~1400

AuthorJun Seok Park, Robin B Guevarra, Bo-Ra Kim, Jun Hyung Lee, Sun Hee Lee, Jae Hyoung Cho, Hyeri Kim, Jin Ho Cho, Minho Song, Ju-Hoon Lee, Richard E Isaacson, Kun Ho Song, Hyeun Bum Kim
Place of dutyCollege of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea
TitleIntestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-9
AbstractCanine parvoviral enteritis (PVE) is an important intestinal disease of the puppies; however, the potential impact of the canine parvovirus (CPV) on the gut microbiota has not been investigated. Therefore, the aim of this study was to evaluate the gut microbial shifts in puppies naturally infected with CPV. Fecal samples were collected from healthy dogs and those diagnosed with PVE at 4, 6, 8, and 12 weeks of age. The distal gut microbiota of dogs was characterized using Illumina MiSeq sequencing of the bacterial 16S rRNA genes. The sequence data were analyzed using QIIME with an Operational Taxonomic Unit definition at a similarity cutoff of 97%. Our results showed that the CPV was associated with significant microbial dysbiosis of the intestinal microbiota. Alpha diversity and species richness and evenness in dogs with PVE decreased compared to those of healthy dogs. At the phylum level, the proportion of Proteobacteria was significantly enriched in dogs with PVE while Bacteroidetes was significantly more abundant in healthy dogs (p < 0.05). In dogs with PVE, Enterobacteriaceae was the most abundant bacterial family accounting for 36.44% of the total bacterial population compared to only 0.21% in healthy puppies. The two most abundant genera in healthy dogs were Prevotella and Lactobacillus and their abundance was significantly higher compared to that of dogs with PVE (p < 0.05). These observations suggest that disturbances of gut microbial communities were associated with PVE in young dogs. Evaluation of the roles of these bacterial groups in the pathophysiology of PVE warrants further studies.
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Supplemental Data
Key_wordGut microbiota, canine parvovirus, dogs, 16S rRNA
References
  1. Nandi S, Kumar M. 2010. Canine parvovirus: current perspective. Indian J. Virol. 21: 31-44.
    Pubmed CrossRef Pubmed Central
  2. Kilian E, Suchodolski JS, Hartmann K, Mueller RS, Wess G, Unterer S. 2018. Long-term effects of canine parvovirus infection in dogs. PLoS One 13: e0192198.
    Pubmed CrossRef Pubmed Central
  3. Sime TA, Powell LL, S childt JC, O lson EJ. 2 0 15. P arvoviral myocarditis in a 5-week-old Dachshund. J. Vet. Emerg. Crit. Care (San Antonio) 25: 765-769.
    Pubmed CrossRef
  4. Tress B, Dorn ES, Suchodolski JS, Nisar T, Ravindran P, Weber K, et al. 2017. Bacterial microbiome of the nose of healthy dogs and dogs with nasal disease. PLoS One 12:e0176736.
    Pubmed CrossRef Pubmed Central
  5. Guevarra RB, Lee JH, Lee SH, Seok MJ, Kim DW, Kang BN, et al. 2019. Piglet gut microbial shifts early in life: causes and effects. J. Anim. Sci. Biotechnol. 10: 1.
    Pubmed CrossRef Pubmed Central
  6. Song ES, Jung SI, Park HJ, Seo KW, Son JH, Hong S, et al. 2016. Comparison of Fecal Microbiota between German Holstein Dairy Cows with and without Left-Sided Displacement of the Abomasum. J. Clin. Microbiol. 54: 1140-1143.
    Pubmed CrossRef Pubmed Central
  7. Kim HB, Isaacson RE. 2017. Salmonella in Swine: Microbiota Interactions. Ann. Rev. Anim. Biosci. 5: 43-63.
    Pubmed CrossRef
  8. Handl S, Dowd SE, Garcia-Mazcorro JF, Steiner JM, Suchodolski JS. 2011. Massive parallel 16S rRNA gene pyrosequencing reveals highly diverse fecal bacterial and fungal communities in healthy dogs and cats. FEMS Microbiol. Ecol. 76: 301-310.
    Pubmed CrossRef
  9. Hooda S, Minamoto Y, Suchodolski JS, Swanson KS. 2012. Current state of knowledge: the canine gastrointestinal microbiome. Anim. Health Res. Rev. 13: 78-88.
    Pubmed CrossRef
  10. Omori M, Maeda S, Igarashi H, Ohno K, Sakai K, Yonezawa T, et al. 2017. Fecal microbiome in dogs with inflammatory bowel disease and intestinal lymphoma. J. Vet. Med. Sci. 79:1840-1847.
    Pubmed CrossRef Pubmed Central
  11. Kalenyak K, Isaiah A, Heilmann RM, Suchodolski JS, Burgener IA. 2018. Comparison of the intestinal mucosal microbiota in dogs diagnosed with idiopathic inflammatory bowel disease and dogs with food-responsive diarrhea before and after treatment. FEMS Microbiol. Ecol. 94(2). doi:10.1093/femsec/fix173.
    Pubmed CrossRef
  12. Song YG, Guevarra RB, Lee JH, Wattanaphansak S, Kang BN, Kim HB, et al. 2017. Comparative analysis of the reproductive tract microbial communities in female dogs with and without pyometra through the 16S rRNA gene pyrosequencing. Jpn. J. Vet. Res. 65: 193-200.
  13. Guevarra RB, Hong SH, Cho JH, Kim BR, Shin J, Lee JH, et al. 2018. The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition. J. Anim. Sci. Biotechnol. 9: 54.
    Pubmed CrossRef Pubmed Central
  14. Hanshew AS, Mason CJ, Raffa KF, Currie CR. 2013. Minimization of chloroplast contamination in 16S rRNA gene pyrosequencing of insect herbivore bacterial communities. J. Microbiol. Methods 95: 149-155.
    Pubmed CrossRef Pubmed Central
  15. Kim T Y, L ee JJ, K im B S, C hoi SH. 20 17. Whole-Body Microbiota of Sea Cucumber (Apostichopus japonicus) from South Korea for Improved Seafood Management. J. Microbiol. Biotechnol. 27: 1753-1762.
    Pubmed CrossRef
  16. Choi S, Hwang YJ, Shin MJ, Yi H. 2017. Difference in the Gut Microbiome between Ovariectomy-Induced Obesity and Diet-Induced Obesity. J. Microbiol. Biotechnol. 27: 2228-2236.
    Pubmed CrossRef
  17. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, et al. 2009. Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities. Appl. Environ. Microbiol. 75: 7537-7541.
    Pubmed CrossRef Pubmed Central
  18. Kim D, Hong S, Kim YT, Ryu S, Kim HB, Lee JH. 2018. Metagenomic Approach to Identifying Foodborne Pathogens on Chinese Cabbage. J. Microbiol. Biotechnol. 28: 227-235.
    Pubmed CrossRef
  19. Kim D, Hong S, Na H, Chun J, Guevarra RB, Kim YT, et al. 2018. Analysis of Microbiota in Bellflower Root, Platycodon grandiflorum, Obtained from South Korea. J. Microbiol. Biotechnol. 28: 551-560.
  20. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7: 335-336.
    Pubmed CrossRef Pubmed Central
  21. Parks D H, T yson G W, H ugenholtz P, Beiko R G. 2014. STAMP: statistical analysis of taxonomic and functional profiles. Bioinformatics 30: 3123-3124.
    Pubmed CrossRef Pubmed Central
  22. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, et al. 2011. Metagenomic biomarker discovery and explanation. Genome Biol. 12(6): R60.
    Pubmed CrossRef Pubmed Central
  23. Luna LG. 1968. Manual of histologic staining methods of the Armed Forces Institute of Pathology. pp. 158-160. 3rd Edition. New York: McGraw-Hill.
  24. Kim BR, Shin J, Guevarra R, Lee JH, Kim DW, Seol KH, et al. 2017. Deciphering Diversity Indices for a Better Understanding of Microbial Communities. J. Microbiol. Biotechnol. 27: 2089-2093.
    Pubmed CrossRef
  25. Moon HS, Lee SA, Lee SG, Choi R, Jeoung SY, Kim D, et al. 2008. Comparison of the pathogenicity in three different Korean canine parvovirus 2 (CPV-2) isolates. Vet. Microbiol. 131: 47-56.
    Pubmed CrossRef
  26. Yildiz S, Mazel-Sanchez B, Kandasamy M, Manicassamy B, Schmolke M. 2018. Influenza A virus infection impacts systemic microbiota dynamics and causes quantitative enteric dysbiosis. Microbiome 6(1): 9.
    Pubmed CrossRef Pubmed Central
  27. Rowan-Nash AD, Korry BJ, Mylonakis E, Belenky P. 2019. Cross-Domain and Viral Interactions in the Microbiome. Microbiol. Mol. Biol. Rev. 83(1). pii: e00044-18.
    Pubmed CrossRef
  28. Zaneveld JR, McMinds R, Vega Thurber R. 2017. Stress and stability: applying the Anna Karenina principle to animal microbiomes. Nat. Microbiol. 2: 17121.
    Pubmed CrossRef
  29. Vazquez-Baeza Y, Hyde ER, Suchodolski JS, Knight R. 2016. Dog and human inflammatory bowel disease rely on overlapping yet distinct dysbiosis networks. Nat. Microbiol. 1: 16177.
    Pubmed CrossRef
  30. Suchodolski JS, Markel ME, G arcia-Mazcorro JF, Unterer S , Heilmann RM, Dowd SE, et al. 2012. The fecal microbiome in dogs with acute diarrhea and idiopathic inflammatory bowel disease. PLoS One 7: e51907.
    Pubmed CrossRef Pubmed Central
  31. Jia J, Frantz N, Khoo C, Gibson GR, Rastall RA, McCartney AL. 2010. Investigation of the faecal microbiota associated with canine chronic diarrhoea. FEMS Microbiol. Ecol. 71: 304-312.
    Pubmed CrossRef
  32. Hullar MAJ, Lampe JW, Torok-Storb BJ, Harkey MA. 2018. The canine gut microbiome is associated with higher risk of gastric dilatation-volvulus and high risk genetic variants of the immune system. PLoS One 13: e0197686.
    Pubmed CrossRef Pubmed Central
  33. Petersen C, Round JL. 2014. Defining dysbiosis and its influence on host immunity and disease. Cell Microbiol. 16:1024-1033.
    Pubmed CrossRef Pubmed Central
  34. Suchodolski JS, Dowd SE, Wilke V, Steiner JM, Jergens AE. 2012. 16S rRNA gene pyrosequencing reveals bacterial dysbiosis in the duodenum of dogs with idiopathic inflammatory bowel disease. PLoS One 7: e39333.
    Pubmed CrossRef Pubmed Central
  35. Guard BC, Suchodolski JS. 2016. HORSE SPECIES SYMPOSIUM: Canine intestinal microbiology and metagenomics:From phylogeny to function. J. Anim. Sci. 94: 2247-2261.
    Pubmed CrossRef
  36. Suchodolski JS, Camacho J, Steiner JM. 2008. Analysis of bacterial diversity in the canine duodenum, jejunum, ileum, and colon by comparative 16S rRNA gene analysis. FEMS Microbiol. Ecol. 66: 567-578.
    Pubmed CrossRef
  37. Heilmann RM, Guard MM, Steiner JM, Suchodolski JS, Unterer S. 2017. Fecal markers of inflammation, protein loss, and microbial changes in dogs with the acute hemorrhagic diarrhea syndrome (AHDS). J. Vet. Emerg. Crit. Care (San Antonio) 27: 586-589.
    Pubmed CrossRef
  38. Cassmann E, White R, Atherly T, Wang C, Sun Y, Khoda S, et al. 2016. Alterations of the Ileal and Colonic Mucosal Microbiota in Canine Chronic Enteropathies. PLoS One 11:e0147321.
    Pubmed CrossRef Pubmed Central
  39. Carding S, Verbeke K, Vipond DT, Corfe BM, Owen LJ. 2015. Dysbiosis of the gut microbiota in disease. Microb. Ecol. Health Dis. 26: 26191.
    Pubmed CrossRef Pubmed Central



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