2019 ; Vol.29-8: 1221~1229
|Author||Wenyong Ding, Houli Zhang, Yuefei Xu, Li Ma, Wenli Zhang|
|Place of duty||Biochemistry and Molecular Biology Department, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, P.R. China|
|Title||Proteomic and Morphologic Evidence for Taurine-5-Bromosalicylaldehyde Schiff Base as an Efficient Anti-Mycobacterial Drug|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Mycobacterium tuberculosis, a causative pathogen of tuberculosis (TB), still threatens human
health worldwide. To find a novel drug to eradicate this pathogen, we tested taurine-5-
bromosalicylaldehyde Schiff base (TBSSB) as an innovative anti-mycobacterial drug using
Mycobacterium smegmatis as a surrogate model for M. tuberculosis. We investigated the
antimicrobial activity of TBSSB against M. smegmatis by plotting growth curves, examined the
effect of TBSSB on biofilm formation, observed morphological changes by scanning electron
microscopy and transmission electron microscopy, and detected differentially expressed
proteins using two-dimensional gel electrophoresis coupled with mass spectrometry. TBSSB
inhibited mycobacterial growth and biofilm formation, altered cell ultrastructure and
intracellular content, and inhibited cell division. Furthermore, M. smegmatis adapted itself to
TBSSB inhibition by regulating the metabolic pathways and enzymatic activities of the
identified proteins. NDMA-dependent methanol dehydrogenase, NAD(P)H nitroreductase,
and amidohydrolase AmiB1 appear to be pivotal factors to regulate the M. smegmatis survival
under TBSSB. Our dataset reinforced the idea that Schiff base-taurine compounds have the
potential to be developed as novel anti-mycobacterial drugs.|
|Key_word||Mycobacterium, tuberculosis, taurine-5-bromosalicylaldehyde Schiff base, morphology, cell wall, two dimensional gel electrophoresis|
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