2019 ; Vol.29-5: 758~764
|Author||Xing Zhou, Jing He, Lingling Wang, Yang Wang, Guocheng Du, Zhen Kang|
|Place of duty||The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Jiangnan University, P.R.China|
|Title||Metabolic Engineering of Saccharomyces cerevisiae to Improve Glucan Biosynthesis|
J. Microbiol. Biotechnol.2019 ;
|Abstract||β-Glucan is a chief structural polymer in the cell wall of yeast. β-Glucan has attracted intensive
attention because of its wide applications in health protection and cosmetic areas. In the
present study, the β-glucan biosynthesis pathway in S. Cerevisiae was engineered to enhance
β-glucan accumulation. A newly identified bacterial β-1, 6-glucan synthase GsmA from
Mycoplasma agalactiae was expressed, and increased β-glucan content by 43%. In addition,
other pathway enzymes were investigated to direct more metabolic flux towards the building
of β-glucan chains. We found that overexpression of Pgm2 (phosphoglucomutase) and Rho1 (a
GTPase for activating glucan synthesis) significantly increased β-glucan accumulation. After
further optimization of culture conditions, the β-glucan content was increased by 53.1%. This
study provides a new approach to enhance β-glucan biosynthesis in Saccharomyces cerevisiae.|
|Key_word||Saccharomyces cerevisiae, β-glucan, synthase, enzyme overexpression, cell resistance|
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