2019 ; Vol.29-4: 587~595
|Author||Changqing Liu, Xing-Chen Wei, Fa-Liang An, Yan-Hua Lu|
|Place of duty||State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, P.R. China|
|Title||Ammonium Acetate Supplement Strategy for Enhancement of Chaetominine Production in Liquid Culture of Marine-Derived Aspergillus fumigatus CY018|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Pharmacological research on (CHA), a marine-derived quinazolinone alkaloid with significant
cytotoxic activity, is restricted by low yields and is a problem that needs to be settled urgently.
In this work, the selection of additional nitrogen sources and the optimization of additional
concentrations and longer fermentation times using ammonium acetate, were investigated.
CHA production was optimized to 62.1 mg/l with the addition of 50 mM ammonium acetate
at 120 h of the fermentation in the shaker flask. This feeding strategy significantly increased 3-
deoxy-arabino-heptulosonate-7-phosphate synthase activity and transcript levels of critical
genes (laeA, dahp and trpC) in the shikimate pathway compared with the non-treatment group.
In addition, the selection of the feeding rate (0.01 and 0.03 g/l/h) was investigated in a 5-L
bioreactor. As a result, CHA production was increased by 57.9 mg/l with a 0.01 g/l/h
ammonium acetate feeding rate. This work shows that the strategy of ammonium acetate
supplementation had an effective role in improving CHA production by Aspergillus fumigatus
CY018. It also shows that this strategy could serve as an important example of large-scale
fermentation of a marine fungus in submerged culture.|
|Key_word||Chaetominine, marine-derived fungus, ammonium acetate, feeding strategy, Aspergillus fumigatus|
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