Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-7: 1078~1082

AuthorSang-Joon Park, Hiroshi Uyama, Mi-Sun Kwak, Monn-Hee Sung
Place of dutyDepartment of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University, Seoul 02707, Republic of Korea
TitleComparison of the Stability of Poly-γ-Glutamate Hydrogels Prepared by UV and γ-Ray Irradiation
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-7
AbstractPoly-γ–glutamate (γ-PGA) has various applications due to its desirable characteristics in terms of safety and biodegradability. Previous studies have been conducted on γ-PGA hydrogels produced by γ-ray irradiation, but these hydrogels have proved unstable in solutions. This study was conducted to enable the γ-PGA hydrogel to maintain a stable form in solutions. The γ-PGA mixture for UV-irradiation was prepared with a cross-linker (N,N,N-trimethyl-3-[(2- methylacryloyl)amino]propan-1-aminium). Both γ-PGA hydrogels’ characteristics, including stability in solutions, were examined. The UV-irradiated γ-PGA hydrogel maintained a stable form during the nine weeks of the study, but the γ-ray irradiated hydrogel dissolved after one week.
Full-Text
Key_wordViscoelasticity, stability, Poly-γ–glutamate, UV irradiated hydrogel, γ-ray irradiated hydrogel, Bacillus sp.
References
  1. Giri TK, Thakur A, Alexander A, Badwaik H, Tripathi DK. 2012. Modified chitosan hydrogels as drug delivery and tissue engineering systems: present status and applications. Acta Pharm. Sin. B. 2: 439-449.
    CrossRef
  2. Lee Y-H, Chang J-J, Yang M-C, Chien C-T, Lai W-F. 2012. Acceleration of wound healing in diabetic rats by layered hydrogel dressing. Carbohydr. Polym. 88: 809-819.
    CrossRef
  3. Azuma C, Yasuda K, Tanabe Y, Taniguro H, Kanaya F, Nakayama A, et al. 2007. Biodegradation of high-toughness double network hydrogels as potential materials for artificial cartilage. J. Biomed. Mater. Res. A. 81: 373-380.
    Pubmed CrossRef
  4. Pan L, Yu G, Zhai D, Lee HR, Zhao W, Liu N, et al. 2012. Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity. Proc. Natl. Acad. Sci. USA 109: 9287-9292.
    Pubmed CrossRef Pubmed Central
  5. Salick DA, Kretsinger JK, Pochan DJ, Schneider JP. 2007. Inherent antibacterial activity of a peptide-based ?-hairpin hydrogel. J. Am. Chem. Soc. 129: 14793-14799.
    Pubmed CrossRef Pubmed Central
  6. Thomas V, Yallapu MM, Sreedhar B, Bajpai S. 2007. A versatile strategy to fabricate hydrogel–silver nanocomposites and investigation of their antimicrobial activity. J. Colloid Interface Sci. 315: 389-395.
    Pubmed CrossRef
  7. Murakami S, Aoki N, Matsumura S. 2011. Bio-based biodegradable hydrogels prepared by crosslinking of microbial poly(γ-glutamic acid) with L-lysine in aqueous solution. Nat. Polymer J. 43: 414-420.
    CrossRef
  8. Lee E-H, Kamigaito Y, Tsujimoto T, SEKI S, UYAMA H, TAGAWA S, et al. 2010. Preparation of Poly (γ-glutamic acid) hydrogel/apatite composites and their application for scaffold of cell proliferation. J. Fiber Sci. Technol. 66: 104-111.
    CrossRef
  9. Chung S, Gentilini C, Callanan A, Hedegaard M, Hassing S, Stevens MM. 2013. Responsive poly (γ-glutamic acid) fibres for biomedical applications. J. Mater. Chem. B. 1: 1397-1401.
    CrossRef
  10. Valliant EM, Romer F, Wang D, McPhail DS, Smith ME, Hanna JV, et al. 2013. Bioactivity in silica/poly (γ-glutamic acid) sol–gel hybrids through calcium chelation. Acta Biomater. 9: 7662-7671.
    Pubmed CrossRef
  11. Garcia JPD, Hsieh M-F, Doma BT, Peruelo DC, Chen I-H, Lee H-M. 2013. Synthesis of gelatin-γ-polyglutamic acidbased hydrogel for the in vitro controlled release of epigallocatechin gallate (EGCG) from Camellia sinensis. Polymers 6: 39-58.
    CrossRef
  12. Sung MH, Park C, Kim CJ, Poo H, Soda K, Ashiuchi M. 2005. Natural and edible biopolymer poly-gamma-glutamic acid: synthesis, production, and applications. Chem. Rec. 5:352-366.
    Pubmed CrossRef
  13. Poo H, Park C, Kwak MS, Choi DY, Hong SP, Lee IH, et al. 2010. New biological functions and applications of highmolecularmass Poly-γ-glutamic acid. Chem. Biodivers. 7:1555-1562.
    Pubmed CrossRef
  14. Ho GH, Ho TI, Hsieh KH, Su YC, Lin PY, Yang J, et al. 2006. γ-Polyglutamic acid produced by Bacillus subtilis (Natto): structural characteristics, chemical properties and biological functionalities. J. Chin. Chem. Soc. 53: 1363-1384.
    CrossRef
  15. Li Z, He G, Hua J, Wu M, Guo W, Gong J, et al. 2017. Preparation of γ-PGA hydrogels and swelling behaviors in salt solutions with different ionic valence numbers. RSC Adv. 7: 11085-11093.
    CrossRef
  16. Choi S-H, Whang K-S, Park J-S, Choi W-Y, Yoon M-H. 2005. Preparation a nd s welling c harac teristic s o f h ydrogel f rom microbial poly (γ-glutamic acid) by γ-irradiation. Macromol. Res. 13: 339-343.
    CrossRef
  17. Uchida R, Sato T, Tanigawa H, Uno K. 2003. Azulene incorporation and release by hydrogel containing methacrylamide propyltrimenthylammonium chloride, and its application to soft contact lens. JCR 92: 259-264.
    CrossRef
  18. Baker JP, Blanch HW, Prausnitz JM. 1995. Swelling properties of acrylamide-based ampholytic hydrogels: comparison of experiment with theory. Polymer 36: 1061-1069.
    CrossRef
  19. Zhang X, Colón LA. 2006. Evaluation of poly {-Nisopropylacrylamideco-[3-(methacryloylamino) propyl] trimethylammonium} as a stationary phase for capillary electrochromatography. Electrophoresis 27: 1060-1068.
    Pubmed CrossRef
  20. Aleksey V. Kurdyumov, Dale G. Swan, et al. 2017. Photoactivatable Crosslinker. U.S. Patent No. 20170022375A1. Surmodies, Inc., Eden Prairie, MN, U.S
  21. Shiladitya SENGUPTA, Suresh Rameshlal CHAWRAI, Shamik GHOSH, Sumana GHOSH, Nilu JAIN, Suresh SADHASIVAM, et al. 2018. Treatments for Resistant Acne. U.S. Patent No. 20160346294A1. IN. New Delhi: Vyome Biosciences Pvt. Ltd.
  22. Dong Wang, Scott C. Miller, et al. 2005. Water-soluble polymeric bone-targeting drug delivery system. U.S. Patent No. 20050287114A1. University of Utah Research Foundation.
  23. Espinosa-Andrews H, Enríquez-Ramírez KE, GarcíaMárquez E, Ramírez-Santiago C, Lobato-Calleros C, VernonCarter J. 2013. Interrelationship between the zeta potential and viscoelastic properties in coacervates complexes. Carbohydr. Polym. 95: 161-166.
    Pubmed CrossRef
  24. Gopinathan J, Noh I. 2018. Recent trends in bioinks for 3D printing. Biomater. Res. 22: 11.
    Pubmed CrossRef Pubmed Central
  25. Ahn J-I, Kuffova L, Merrett K, Mitra D, Forrester JV, Li F, et al. 2013. Crosslinked collagen hydrogels as corneal implants:effects of sterically bulky vs. non-bulky carbodiimides as crosslinkers. Acta Biomater. 9: 7796-7805.
    Pubmed CrossRef
  26. Arakaki K, Kitamura N, Fujiki H, Kurokawa T, Iwamoto M, Ueno M, et al. 2010. Artificial cartilage made from a novel double-network hydrogel: in vivo effects on the normal cartilage and ex vivo evaluation of the friction property. J. Biomed. Mater. Res. A. 93: 1160-1168.
    Pubmed CrossRef



Copyright © 2009 by the Korean Society for Microbiology and Biotechnology.
All right reserved. Mail to jmb@jmb.or.kr
Online ISSN: 1738-8872    Print ISSN: 1017-7825    Powered by INFOrang Co., Ltd