2019 ; Vol.29-4: 607~616
|Author||Han Li, Xiumei Cui, Liangyu Zheng|
|Place of duty||Jilin University, People’s Republic of China. |
|Title||Functionalized Poplar Powder as a Support Material for Immobilization of Enoate Reductase and a Cofactor Regeneration System|
J. Microbiol. Biotechnol.2019 ;
|Abstract||In this study, functionalized poplar powder (FPP) was used as a support material for the
immobilization of enoate reductase (ER) and glucose-6-phosphate dehydrogenase (GDH) by
covalent binding. Under optimal conditions, the immobilization efficiency of ER-FPP and
GDH-FPP was 95.1% and 84.7%, and the activity recovery of ER and GDH was 47.5% and
37.8%, respectively. Scanning electron microscopy (SEM) and energy dispersive spectroscopy
(EDS) analysis indicated that FPP was a suitable carrier for enzyme immobilization. ER-FPP
and GDH-FPP exhibit excellent thermal stabilities and superior reusability. Especially, ER-FPP
and GDH-FPP enable the continuous conversion of 4-(4-Methoxyphenyl)-3-buten-2-one with
NAD+ recycling. While the immobilization strategies established here were simple and
inexpensive, they exploited a new method for the immobilization and application of ER and its
cofactor recycling system.|
|Key_word||Enoate reductase, functionalized poplar powder, covalent immobilization, cofactor regeneration system|
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