2019 ; Vol.29-2: 230~234
|Author||Yunlong Sun, Yali Niu, Bin He, Long Ma, Ganghua Li, Van-Tuan Tran, Bin Zeng, Zhihong Hu|
|Place of duty||Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal University|
|Title||A Dual Selection Marker Transformation System Using Agrobacterium tumefaciens for the Industrial Aspergillus oryzae 3.042|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplastmediated
transformation (PMT). In this study, we established a dual selection marker system
in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated
transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042
strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT
system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers.
Finally, a dual selection marker ATMT system was developed. This study demonstrates a
useful dual selection marker transformation system for genetic manipulations of A. oryzae
|Key_word||Aspergillus oryzae, uridine/uracil auxotrophy, pyrithiamine, Agrobacterium tumefaciens-mediated transformation|
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