Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-2: 230~234

AuthorYunlong Sun, Yali Niu, Bin He, Long Ma, Ganghua Li, Van-Tuan Tran, Bin Zeng, Zhihong Hu
Place of dutyJiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal University
TitleA Dual Selection Marker Transformation System Using Agrobacterium tumefaciens for the Industrial Aspergillus oryzae 3.042
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-2
AbstractCurrently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplastmediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.
Full-Text
Supplemental Data
Key_wordAspergillus oryzae, uridine/uracil auxotrophy, pyrithiamine, Agrobacterium tumefaciens-mediated transformation
References
  1. Yamada R, Yoshie T, Wakai S, Asai-Nakashima N, Okazaki F, Ogino C. et al. 2014. Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose. Microb. Cell Fact. 13: 71.
    Pubmed CrossRef Pubmed Central
  2. Kitamoto K. 2015. Cell biology of the Koji mold Aspergillus oryzae. Biosci. Biotech. Bioch. 79: 863-869.
    Pubmed CrossRef
  3. Wang B, Guo G, Wang C, Lin Y, Wang X, Zhao M, et al. 2010. Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing. Nucleic. Acids Res. 38:5075-5087.
    Pubmed CrossRef Pubmed Central
  4. Ichishima E. 2016. Development of enzyme technology for Aspergillus oryzae, A. sojae, and A. luchuensis, the national microorganisms of Japan. Biosci. Biotech. Bioch. 80: 1681-1692.
    Pubmed CrossRef
  5. Suzuki S, Tada S, Fukuoka M, Taketani H, Tsukakoshi Y, Matsushita M, et al. 2009. A novel transformation system using a bleomycin resistance marker with chemosensitizers for Aspergillus oryzae. Biochem. Biophys. Res. Commun. 383:42-47.
    Pubmed CrossRef
  6. Gomi K., Kitamoto, K., & Kumagai, C. 1992. Transformation of the industrial strain of Aspergillus oryzae, with the homologous amds, gene as a dominant selectable marker. J. Ferment. Bioeng. 74: 389-391.
    CrossRef
  7. Lubertozzi D, Keasling JD. 2006. Marker and promoter effects on heterologous expression in Aspergillus nidulans. Appl. Microbiol. Biotechnol. 72: 1014-1023.
    Pubmed CrossRef
  8. Ling SOS, Storms R, Zheng Y, Rodzi MRM, Mahadi NM, Illias RM, et al. 2013. Development of a pyrG mutant of Aspergillus oryzae strain S1 as a host for the production of heterologous proteins. Scientific World J. 2013: 1-7.
    Pubmed CrossRef Pubmed Central
  9. Nguyen KT, Ho QN, Do L, Mai LTD, Pham DN, Tran HTT, et al. 2017. A new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus Aspergillus oryzae using Agrobacterium tumefaciensmediated transformation. World J. Microbiol. Biotechnol. 33: 107.
    Pubmed CrossRef
  10. Jin FJ, Maruyama J, Juvvadi PR, Arioka M, Kitamoto K. 2004. Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae. FEMS Microbiol. Lett. 239: 79-85.
    Pubmed CrossRef
  11. Jin FJ, Maruyama J, Juvvadi PR, Arioka M, Kitamoto K. 2004. Adenine auxotrophic mutants of aspergillus oryzae:development of a novel transformation system with triple auxotrophic hosts. Biosci. Biotechnol. Biochem. 68: 656-662.
    Pubmed CrossRef
  12. Du Y, Xie G, Yang C, Fang B, Chen H. 2014. Construction of brewing-wine Aspergillus oryzae pyrG- mutant by pyrG gene deletion and its application in homology transformation. Acta Biochim. Biophys. Sin. 46: 477-483.
    Pubmed CrossRef
  13. Kubodera T, Yamashita N, Nishimura A. 2000. Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. Biosci. Biotechnol. Biochem. 64:1416-1421.
    Pubmed CrossRef
  14. Kubodera T, Yamashita N, Nishimura A. 2002. Transformation of Aspergillus sp. and Trichoderma reesei using the pyrithiamine resistance gene (ptrA) of Aspergillus oryzae. Biosci. Biotechnol. Biochem. 66: 404-406.
    Pubmed CrossRef
  15. Weyda I, Yang L, Vang J, Ahring BK, Lübeck M, Lübeck PS. 2017. A comparison of Agrobacterium-mediated transformation and protoplast-mediated transformation with crispr-cas9 and bipartite gene targeting substrates, as effective gene targeting tools for Aspergillus carbonarius. J. Microbiol. Methods 135: 26-34.
    Pubmed CrossRef
  16. Idnurm A, Bailey AM, Cairns TC, Elliott CE, Foster GD, Ianiri G, et al. 2017. A silver bullet in a golden age of functional genomics: the impact of Agrobacterium-mediated transformation of fungi. Fungal Biol. Biotechnol. 4: 6.
    Pubmed CrossRef Pubmed Central
  17. Nguyen KT, Ho QN, Pham TH, Phan TN, Tran VT. 2016. The construction and use of versatile binary vectors carrying pyrG auxotrophic marker and fluorescent reporter genes for Agrobacterium-mediated transformation of Aspergillus oryzae. World J. Microbiol. Biotechnol. 32: 204.
    Pubmed CrossRef
  18. Ling SO, Storms R, Zheng Y, Rodzi MR, Mahadi NM, Illias RM, et al. 2013. Development of a pyrG mutant of Aspergillus oryzae strain S1 as a host for the production of heterologous proteins. Scientific World J. 2013: 634317.
    Pubmed CrossRef Pubmed Central



Copyright © 2009 by the Korean Society for Microbiology and Biotechnology.
All right reserved. Mail to jmb@jmb.or.kr
Online ISSN: 1738-8872    Print ISSN: 1017-7825    Powered by INFOrang.co., Ltd