2019 ; Vol.29-2: 200~208
|Author||Whee-Soo Kim, Chong-Su Cho, Liang Hong, Geon Goo Han, Bum Ju Kil, Sang-Kee Kang, Dae-Duk Kim, Yun-Jaie Choi, Chul-Sung Huh|
|Place of duty||Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea|
|Title||Oral Delivery of Probiotics Using pH-Sensitive Phthalyl Inulin Tablets|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Probiotics show low cell viability after oral administration because they have difficulty
surviving in the stomach due to low pH and enzymes. For the oral delivery of probiotics,
developing a formula that protects the probiotic bacteria from gastric acidity while providing
living cells is mandatory. In this study, we developed tablets using a new pH-sensitive
phthalyl inulin (PI) to protect probiotics from gastric conditions and investigated the effects of
different compression forces on cell survival. We made three different tablets under different
compression forces and measured survivability, disintegration time, and kinetics in simulated
gastric-intestinal fluid. During tableting, there were no significant differences in probiotic
viability among the different compression forces although disintegration time was affected by
the compression force. A higher compression force resulted in higher viability in simulated
gastric fluid. The swelling degree of the PI tablets in simulated intestinal fluid was higher than
that of the tablets in simulated gastric fluid due to the pH sensitivity of the PI. The probiotic
viability formulated in the tablets was also higher in acidic gastric conditions than that for
probiotics in solution. Rapid release of the probiotics from the tablet occurred in the simulated
intestinal fluid due to the pH sensitivity. After 6 months of refrigeration, the viability of the PI
probiotics was kept. Overall, this is the first study to show the pH-sensitive properties of PI
and one that may be useful for oral delivery of the probiotics.|
|Key_word||Probiotics, oral delivery, pH-sensitive tablet, phthalyl inulin|
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