Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-3: 465~472

AuthorHyunjin Hong, Yeonhee Lee
Place of dutyCulture Collection of Antimicrobial Resistant Microbes, Republic of Korea,Department of Horticulture, Biotechnology, and Landscape Architecture, Seoul Women’s University, Republic of Korea
TitleTransfer of Antimicrobial-Resistant Escherichia coli and Resistance Genes in a Child Care Center
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-3
AbstractSeveral reports describe antimicrobial-resistance transfer among children and the community in outbreak situations, but transfer between a child and a caregiver has not been examined in child care facilities under normal circumstances. We investigated the transfer of antimicrobialresistance genes, resistant bacteria, or both among healthy children and teachers. From 2007 to 2009, 104 Escherichia coli isolates were obtained from four teachers and 38 children in a child care center. Twenty-six cephem-resistant isolates were obtained from children in 2007 and 2008. In 2009, cephem-resistant isolates were detected in children as well as a teacher. Nalidixic acid–resistant isolates from the same teacher for 3 years showed low similarity (<50%) to each other. However, an isolate from a teacher in 2007 and another from a child in 2008 showed high similarity (87%). Pulsed-field gel electrophoresis revealed 100% similarity for four isolates in 2007 and one isolate in 2008, and also similarity among seven isolates carrying the virulence gene (CNF1). This study yielded the following findings: (1) a gene for extended-spectrum β-lactamase was transferred from a child to other children and a teacher; (2) a nalidixic acid–resistant isolate was transferred from a teacher to a child; and (3) a virulent bacterium was transferred between children.
Full-Text
Key_wordEscherichia coli, child care center, antimicrobial resistance, transfer
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