2019 ; Vol.29-3: 465~472
|Author||Hyunjin Hong, Yeonhee Lee|
|Place of duty||Culture Collection of Antimicrobial Resistant Microbes, Republic of Korea,Department of Horticulture, Biotechnology, and Landscape Architecture, Seoul Women’s University, Republic of Korea|
|Title||Transfer of Antimicrobial-Resistant Escherichia coli and Resistance Genes in a Child Care Center|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Several reports describe antimicrobial-resistance transfer among children and the community
in outbreak situations, but transfer between a child and a caregiver has not been examined in
child care facilities under normal circumstances. We investigated the transfer of antimicrobialresistance
genes, resistant bacteria, or both among healthy children and teachers. From 2007 to
2009, 104 Escherichia coli isolates were obtained from four teachers and 38 children in a child
care center. Twenty-six cephem-resistant isolates were obtained from children in 2007 and
2008. In 2009, cephem-resistant isolates were detected in children as well as a teacher.
Nalidixic acid–resistant isolates from the same teacher for 3 years showed low similarity
(<50%) to each other. However, an isolate from a teacher in 2007 and another from a child in
2008 showed high similarity (87%). Pulsed-field gel electrophoresis revealed 100% similarity
for four isolates in 2007 and one isolate in 2008, and also similarity among seven isolates
carrying the virulence gene (CNF1). This study yielded the following findings: (1) a gene for
extended-spectrum β-lactamase was transferred from a child to other children and a teacher;
(2) a nalidixic acid–resistant isolate was transferred from a teacher to a child; and (3) a virulent
bacterium was transferred between children.|
|Key_word||Escherichia coli, child care center, antimicrobial resistance, transfer|
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