Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-3: 392~400

AuthorFangyu Cheng, Sijin Luozhong, Huimin Yu, Zhigang Guo
Place of dutyDepartment of Chemical Engineering, Tsinghua University, China,Key Laboratory of Industrial Biocatalysis (Tsinghua University), the Ministry of Education, China
TitleBiosynthesis of Chondroitin in Engineered Corynebacterium glutamicum
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-3
AbstractChondroitin, the precursor of chondroitin sulfate, which is an important polysaccharide, has drawn significant attention due to its applications in many fields. In the present study, a heterologous biosynthesis pathway of chondroitin was designed in a GRAS (generally recognized as safe) strain C. glutamicum. CgkfoC and CgkfoA genes with host codon preference were synthesized and driven by promoter Ptac, which was confirmed as a strong promoter via GFPuv reporter assessment. In a lactate dehydrogenase (ldh) deficient host, intracellular chondroitin titer increased from 0.25 to 0.88 g/l compared with that in a wild-type host. Moreover, precursor enhancement via overexpressing precursor synthesizing gene ugdA further improved chondroitin titers to 1.09 g/l. Chondroitin production reached 1.91 g/l with the engineered strain C. glutamicum ΔL-CgCAU in a 5-L fed-batch fermentation with a single distribution Mw of 186 kDa. This work provides an alternative, safe and novel means of producing chondroitin for industrial applications.
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Key_wordChondroitin biosynthesis, engineered Corynebacterium glutamicum, lactate dehydrogenase deficient, precursor enhancement, fed-batch fermentation
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