Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-2: 311~320

AuthorFang Wang, Wu Qiao, Bin Bao, Shujun Wang, Joe Mac Regenstein, Yile Shi, Wenhui Wu, Ming Ma
Place of dutyShanghai Ocean University, P.R. China
TitleEffect of IgY on Periodontitis and Halitosis Induced by Fusobacterium nucleatum
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-2
AbstractFusobacterium nucleatum is a morbific agent in periodontitis and halitosis. Egg yolk antibody (IgY) was obtained from egg yolks from chickens stimulated with F. nucleatum. This study was to assess the effectiveness of IgY on periodontitis and halitosis caused by F. nucleatum in vitro and in vivo. The growth of F. nucleatum was inhibited (p <0. 05) by different concentrations of IgY in vitro and the results of a Halimeter show volatile sulfur compounds (VSCs) were reduced to 904 ± 57 ppb at a concentration 40 mg/ml of IgY. The changes of fatty acids of F. nucleatum were determined using GC-MS. The scores for odor index of rat saliva were decreased. The major constituent of volatile organic compounds (VOCs) including short-chain acids decreased 46.2% in 10 mg/ml IgY, ammonia decreased 70% in 40 mg/ml IgY, while aldehydes and olefine ketones were almost unchanged. The ELISA assay revealed that IL-6 and TNF-α were decreased after 4 weeks’ IgY treatment. Morphometric (X-ray) and histological analyses (HE) showed that IgY reduced alveolar bone loss and collagen fibers became orderly in rat models. As a result, IgY may have the potential to treat periodontitis and halitosis.
Full-Text
Key_wordEgg Yolk antibody, Fusobacterium nucleatum, halitosis, inflammation, alveolar bone loss
References
  1. Amou T, Hinode D, Yoshioka M, Grenier D. 2014. Relationship between halitosis and periodontal disease - associated oral bacteria. Int. J. Dent. Hyg. 12: 145-151.
    Pubmed CrossRef
  2. Goldberg S, Kozlovsky A, Gordon D, Gelernter I. Sintov A, Rosenberg M. 1994. Cadaverine as a putative component of oral malodor. J. Dent. Res. 73: 1168-1172.
    Pubmed CrossRef
  3. Phillips M, Herrera J, Krishnan S, Zain M, Greenberg J, Cataneo RN. 1999. Variation in volatile organic compounds in the breath of normal humans. J. Chromatogr. B. Biomed. Sci. Appl. 729: 75-88.
    CrossRef
  4. Oh TJ, Eber R, Wang HL. 2002. Periodontal diseases in the child and adolescent. J. Clin. Periodontol. 29: 400-410.
    Pubmed CrossRef
  5. Kolenbrander PE, Andersen RN, Blehert DS, Egland PG, Foster JS, Palmer RJ Jr. 2002. Communication among oral bacteria. Microbiol. Mol. Biol. Rev. 66: 486-505.
    Pubmed CrossRef Pubmed Central
  6. Sato H, Ohkushi T, Kaizu T, Tsunoda M, Sato T. 1980. A study of the mechanism of halitosis occurrence in periodontal patients. Bul. Tokyo Dent. Coll. 21: 271-278.
  7. Yaegaki K, Sanada K. 1992. Biochemical and clinical factors influencing oral malodor in. periodontal. J. Periodontol. 63:783-789.
    Pubmed CrossRef
  8. Kang MS, Kim BG, Chung J, Lee HC, Oh JS. 2006. Inhibitory effect of Weissella cibaria isolates on the production of volatile. J. Clin. Periodontol. 33: 226-232.
    Pubmed CrossRef
  9. Signat B, Roques C, Poulet P, Duffaut D. 2011. Fusobacterium nucleatum in periodontal health and disease. Curr. Issues. Mol. Biol. 13: 25-36.
    Pubmed
  10. Han YW, Shi W, Huang GT, Kinder Haake S, Park NH, Kuramitsu H, et al. 2000. Interactions between periodontal bacteria and human oral epithelial cells. Infect. Immun. 68:3140-3146.
    Pubmed CrossRef Pubmed Central
  11. Handal T, Caugant DA, Olsen I. 2003. Antibiotic resistance in bacteria isolated from subgingival plaque in a norwegian population with refractory marginal periodontitis. Antimicrob. Agents Chemother. 47: 1443-1446.
    Pubmed CrossRef Pubmed Central
  12. Lu Y, Liu J, Jin L. 2009. Passive immunization of crayfish (Procambius clarkiaii) with chicken egg yolk. Appl. Biochem. Biotechnol. 159: 750-758.
    Pubmed CrossRef
  13. Otake S, Nishihara Y, Makimura M, Hatta H, Kim M, Yamamoto T, et al. 1991. Protection of rats against dental caries by passive immunization with hen-egg-yolk antibody (IgY). J. Dent. Res. 70: 162-166.
    Pubmed CrossRef
  14. Hatta H, Tsuda K, Ozeki M, Kim M, Yamamoto T, Otake S, et al. 1997. Passive immunization against dental plaque formation in humans: effect of a mouth rinse containing egg yolk antibodies (IgY) specific to Streptococcus mutans. Caries Res. 31: 268-274.
    Pubmed CrossRef
  15. Hamajima S, Maruyama M, Hijiya T, Hatta H, Abiko Y. 2007. Egg yolk-derived immunoglobulin (IgY) against Porphyromonas gingivalis 40-kDa outer membrane protein inhibits coaggregation activity. Arch. Oral Biol. 52: 697-704.
    Pubmed CrossRef
  16. Li X, Liu H, Xu Y, Xu F, Wang L, You J, et al. 2012. Chicken egg yolk antibody (IgY) controls Solobacterium moorei under in vitro and in vivo conditions. Appl. Biochem. Biotechnol. 168: 1448-1458.
    Pubmed CrossRef
  17. Xu FX, Xu YP, Jin LJ, Liu H, Wang LH, You JS, et al. 2012. Effectiveness of egg yolk immunoglobulin (IgY) against periodontal. J. Appl. Microbiol. 113: 983-991.
    Pubmed CrossRef
  18. Wong L, Sissons C. 2001. A comparison of human dental plaque microcosm biofilms grown in an undefined medium and a chemically defined artificial saliva. Arch. Oral Biol. 46:477-486.
    CrossRef
  19. He Z, Huang Z, Zhou W, Tang Z, Ma R, Liang J. 2016. Antibiofilm activities from resveratrol against Fusobacterium nucleatum. Front. Microbiol. 7: 1065.
    Pubmed CrossRef Pubmed Central
  20. Furne J, Majerus G, Lenton P, Springfield J, Levitt DG, Levitt MD. 2002. Comparison of volatile sulfur compound concentrations measured with a sulfide detector vs. gas chromatography. J. Dent. Res. 81: 140-143.
    Pubmed CrossRef
  21. Greenman J, El-Maaytah M, Duffield J, Spencer P, Rosenberg M, Corry D, et al. 2005. Assessing the relationship between concentrations of malodor compounds and odor scores from judges. J. Am. Dent. Assoc. 136: 749-757.
    Pubmed CrossRef
  22. Lee EN, Sunwoo HH, Menninen K, Sim JS. 2002. In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium. Poult. Sci. 81: 623641.
    Pubmed CrossRef
  23. Chen X, Yang B, Qi C, Sun TW, Chen F, Wu J, et al. 2016. DNA-templated microwave-hydrothermal synthesis of nanostructured hydroxyapatite for storing and sustained release of an antibacterial protein. Dalton Trans. 45: 16481656.
    Pubmed CrossRef
  24. Cho E, Park SN, Lim YK, Shin Y, Paek J, Hwang CH, et al. 2015. Fusobacterium hwasookii sp. nov., isolated from a human periodontitis lesion. Curr. Microbiol. 70: 169-175.
    Pubmed CrossRef
  25. Tonzetich J. 1971. Direct gas chromatographic analysis of sulphur compounds in mouth air in man. Arch. Oral. Biol. 16: 587-597.
    CrossRef
  26. Yoshimura M, Nakano Y, Fukamachi H, Koga T. 2002. 3Chloro-DL-alanine resistance by L-methionine-alpha-deaminogammamercaptomethane-lyase activity. FEBS Lett. 523: 119122.
    CrossRef
  27. Suzuki N, Nakano Y, Watanabe T, Yoneda M, Hirofuji T, Hanioka T. 2018. Two mechanisms of oral malodor inhibition by zinc ions. J. Appl. Oral Sci. 26: e20170161.
    Pubmed CrossRef Pubmed Central
  28. Yoshida Y, Ito S, Kamo M, Kezuka Y, Tamura H, Kunimatsu K, et al. 2010. Production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586. Microbiology 156 (Pt 7): 2260-2269.
    Pubmed CrossRef
  29. Liu J, Ling JQ, Wu CD. 2013. Cetylpyridinium chloride suppresses gene expression associated with halitosis. Arch. Oral Biol. 58: 1686-1691.
    Pubmed CrossRef
  30. Amann A, Costello Bde L, Miekisch W, Schubert J, Buszewski B, Pleil J, et al. 2014. The human volatilome:volatile organic compounds (VOCs) in exhaled breath, skin emanations, urine, feces and saliva. J. Breath. Res. 8: 034001.
    Pubmed CrossRef
  31. Oz HS, Puleo DA. Animal models for periodontal disease. 2011. J. Biomed. Biotechnol. 2011: 754857.
    Pubmed Pubmed Central
  32. Oliveira GJ, Paula LG, Souza JA, Spin-Neto R, Stavropoulos A, Marcantonio RA. 2017. Effects of avocado/soybean unsaponifiables (ASU) on the treatment of ligature-induced periodontitis in rats. Braz. Oral Res. 31: 1-11.
    Pubmed CrossRef
  33. Yokoyama K, Sugano N, Shimada T, Shofiqur RA, Ibrahim el-SM, Isoda R, et al. 2007. Effects of egg yolk antibody against Porphyromonas gingivalis gingipains in periodontitis patients. J. Oral Sci. 49: 201-206.
    Pubmed CrossRef
  34. Iwasaki Y, Otsuka H, Yanagisawa N, Hisamitsu H, Manabe A, Nonaka N, et al. 2011. In situ proliferation and differentiation of macrophages in dental pulp. Cell Tissue Res. 346: 99-109.
    Pubmed CrossRef Pubmed Central
  35. Mosser DM, Edwards JP. 2008. Exploring the full spectrum of macrophage activation. Nat. Rev. Immunol. 10: 958-969.
    Pubmed CrossRef Pubmed Central
  36. Grenier D, Mayrand D. 1985. Cytotoxic effects of culture supernatants of oral. bacteria and various organic. Can. J. Microbiol. 31: 302-304.
    Pubmed CrossRef
  37. Tsuda H, Ochiai K, Suzuki N, Otsuka K. 2010. Butyrate, a bacterial metabolite, induces. apoptosis and autophagic cell death in gingival epithelial cells. J. Periodontal Res. 45: 626634.
    Pubmed CrossRef
  38. Kirschbaum M, Schultze-Mosgau S, Pfister W, Eick S. 2010. Mixture of periodontopathogenic bacteria influences interaction with KB cells. Anaerobe 16: 461-468.
    Pubmed CrossRef
  39. Roberts GL. 2000. Fusobacterial infections: an underestimated threat. Br. J. Biomed. Sci. 57: 156-162.
    Pubmed



Copyright © 2009 by the Korean Society for Microbiology and Biotechnology.
All right reserved. Mail to jmb@jmb.or.kr
Online ISSN: 1738-8872    Print ISSN: 1017-7825    Powered by INFOrang.co., Ltd