2019 ; Vol.29-4: 645~650
|Author||Ji Soo Kim, Won Kon Kim, Kyoung-Jin Oh, Eun-Woo Lee, Baek Soo Han, Sang Chul Lee, Kwang-Hee Bae|
|Place of duty||Metabolic Regulation Research Center, Division of BioMedical Sciences, KRIBB, Daejeon 34141, Republic of Korea,Department of Functional Genomics, University of Science and Technology (UST) of Korea, Daejeon 34141, Republic of Korea|
|Title||Protein Tyrosine Phosphatase, Receptor Type B (PTPRB) Inhibits Brown Adipocyte Differentiation through Regulation of VEGFR2 Phosphorylation|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Brown adipocytes have an important role in the regulation of energy balance through
uncoupling protein-1 (UCP-1)-mediated nonshivering thermogenesis. Although brown
adipocytes have been highlighted as a new therapeutic target for the treatment of metabolic
diseases, such as obesity and type II diabetes in adult humans, the molecular mechanism
underlying brown adipogenesis is not fully understood. We recently found that protein
tyrosine phosphatase receptor type B (PTPRB) expression dramatically decreased during
brown adipogenic differentiation. In this study, we investigated the functional roles of PTPRB
and its regulatory mechanism during brown adipocyte differentiation. Ectopic expression of
PTPRB led to a reduced brown adipocyte differentiation by suppressing the tyrosine
phosphorylation of VEGFR2, whereas a catalytic inactive PTPRB mutant showed no effects on
differentiation and phosphorylation. Consistently, the expression of brown adipocyte-related
genes, such as UCP-1, PGC-1α, PRDM16, PPAR-γ, and CIDEA, were significantly inhibited by
PTPRB overexpression. Overall, these results suggest that PTPRB functions as a negative
regulator of brown adipocyte differentiation through its phosphatase activity-dependent
mechanism and may be used as a target protein for the regulation of obesity and type II
|Key_word||Brown adipogenesis, obesity, PTPRB, VEGFR2|
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