2019 ; Vol.29-4: 596~606
|Author||Wensong Jin, Hui Lin, Huifang Gao, Zewang Guo, Jiahuan Li, Quanming Xu, Shujing Sun, Kaihui Hu, Jung-Kul Lee, Liaoyuan Zhang|
|Place of duty||College of Life Sciences, Fujian Agriculture and Forestry University, P.R. China,Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, P.R. China|
|Title||N-Acyl-Homoserine Lactone Quorum Sensing Switch from Acidogenesis to Solventogenesis during the Fermentation Process in Serratia marcescens MG1|
J. Microbiol. Biotechnol.2019 ;
|Abstract||N-acyl-homoserine lactone quorum sensing (AHL-QS) has been shown to regulate many
physiological behaviors in Serratia marcescens MG1. In the current study, the effects of AHL-QS
on the biosynthesis of acid and neutral products by S. marcescens MG1 and its isogenic ΔswrI
with or without supplementing exogenous N-hexanoyl-L-homoserine lactone (C6-HSL) were
systematically investigated. The results showed that swrI disruption resulted in rapid pH
drops from 7.0 to 4.8, which could be restored to wild type by supplementing C6-HSL.
Furthermore, fermentation product analysis indicated that ΔswrI could lead to obvious
accumulation for acidogenesis products such as lactic acid and succinic acid, especially excess
acetic acid (2.27 g/l) produced at the early stage of fermentation, whereas solventogenesis
products by ΔswrI appeared to noticeably decrease by an approximate 30% for acetoin during
32-48 h and by an approximate 20% for 2,3-butanediol during 24-40 h, when compared to
those by wild type. Interestingly, the excess acetic acid produced could be removed in an
AHL-QS-independent manner. Subsequently, quantitative real-time PCR was used to
determine the mRNA expression levels of genes responsible for acidogenesis and
solventogenesis and showed consistent results with those of product synthesis. Finally, by
close examination of promoter regions of the analyzed genes, four putative luxI box-like
motifs were found upstream of genes encoding acetyl-CoA synthase, lactate dehydrogenase,
α-acetolactate decarboxylase, and Lys-like regulator. The information from this study
provides a novel insight into the roles played by AHL-QS in switching from acidogenesis to
solventogenesis in S. marcescens MG1.|
|Key_word||Serratia marcescens MG1, N-acyl-homoserine lactone quorum sensing, acidogenesis, solventogenesis, switch|
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