2019 ; Vol.29-3: 482~488
|Author||Bo-Kyoung Jung, Hye-Ran Kim, Young-Hyeon Lee, Hyun Jang, Kyung-Soo Chang|
|Place of duty||Catholic University of Pusan, Republic of Korea|
|Title||Comparison of Immune Responses to the PCV2 Replicase-Capsid and Capsid Virus-Like Particle Vaccines in Mice|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting
syndrome (PMWS) in pigs. Replicase (Rep) proteins are considered essential for viral replication.
Capsid (Cap) protein is the primary immunogenic protein that induces protective immunity.
Little is known about comparison on the immunogenicity of PCV2 Rep and Cap fusion protein
and Cap protein. In the present study, recombinant baculoviruses expressing the Rep-Cap
fusion protein (Bac-Rep-Cap) and the Cap protein (Bac-Cap) of PCV2 were constructed and
confirmed with western blot and indirect fluorescence assay. Immunogenicities of the two
recombinant proteins were tested in mice. The titers of antibodies were determined with a
PCV2-specific enzyme-linked immunosorbent assay (ELISA) and a serum neutralization assay.
The IFN-γ response of immunized mice was measured by ELISA. The mice immunized with
the Bac-Rep-Cap and Bac-Cap successfully produced Cap-specific immunoreaction. The mice
immunized with the Bac-Cap developed higher PCV2-specific neutralizing antibody titers than
mice injected with the Bac-Rep-Cap. IFN-γ in the Bac-Rep-Cap group was increased compared
to those in the Bac-Cap group. Vaccination of mice with the Bac-Rep-Cap showed significantly
decreased protective efficacy compared to the Bac-Cap. Our findings will indubitably not only
lead to a better understanding of the immunogenicity of PCV2, but also improved vaccines.|
|Key_word||Baculovirus, capsid, post-weaning multisystemic wasting syndrome, Porcine circovirus type- 2, replicase, vaccine|
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