2019 ; Vol.29-5: 687~695
|Author||Joon Ha Lee, Minchul Seo, Hwa Jeong Lee, Minhee Baek, In Woo Kim, Sun Young Kim, Mi Ae Kim, Seong Hyun Kim, Jae Sam Hwang|
|Place of duty||National Institute of Agricultural Sciences, Republic of Korea|
|Title||Anti-Inflammatory Activity of Antimicrobial Peptide Allomyrinasin Derived from the Dynastid Beetle, Allomyrina dichotoma|
J. Microbiol. Biotechnol.2019 ;
|Abstract||In a previous work, we performed de novo RNA sequencing of Allomyrina dichotoma using next
generation sequencing and identified several antimicrobial peptide candidates based on
transcriptome analysis. Among them, a cationic antimicrobial peptide, allomyrinasin, was
selected bioinformatically based on its physicochemical properties. Here, we assessed the
antimicrobial and anti-inflammatory activities of allomyrinasin against microorganisms and
mouse macrophage Raw264.7 cells. Allomyrinasin showed antimicrobial activities against
various microbes and decreased the nitric oxide production of the lipopolysaccharide-induced
Raw264.7 cells. Furthermore, quantitative RT-PCR and ELISA revealed that allomyrinasin
reduced cytokine expression levels in the Raw264.7 cells. We also identified inducible nitric
oxide synthase, cyclooxygenase-2 expression, and PGE2 production through western blot
analysis and ELISA. We confirmed that allomyrinasin bound to bacterial cell membranes via a
specific interaction with lipopolysaccharides. Taken together, these data indicate that
allomyrinasin has antimicrobial and anti-inflammatory activities as exemplified in
lipopolysaccharide-induced Raw264.7 cells. We have provided a potentially useful
antimicrobial peptide candidate that has both antimicrobial and anti-inflammatory activities.|
|Key_word||Antimicrobial peptide, anti-inflammatory activity, inflammation, lipopolysaccharide, Allomyrina dichotoma|
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