Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2019 ; Vol.29-1: 55~58

AuthorJuyoung Byun, Sena Yoon, Yunji Jeong, Uitaek Oh, Sujin Cho, Jeongsoo Park, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon
Place of dutyPanGen Biotech Inc, Republic of Korea
TitleEfficient Development of Stable Recombinant Chinese Hamster Ovary (rCHO) Cell Lines to Produce Antibodies by Using Dimethyl Sulfoxide (DMSO) in Electroporation
PublicationInfo J. Microbiol. Biotechnol.2019 ; Vol.29-1
AbstractDevelopment of stable rCHO cell lines is still time consuming and labor intensive, although it is a critical step in the commercial development of recombinant antibodies. The current work demonstrates, for the first time, that electroporation of CHO cells with DMSO can enhance stable expression of recombinant antibodies in rCHO cells. Electroporation with DMSO resulted in an average 3.7-fold and 2.8-fold increases in expression levels of aflibercept and pembrolizumab, respectively, in pools of stable rCHO cells. It also resulted in an average of 2.2-fold and 2.6-fold increases in the expression of aflibercept and pembrolizumab, respectively, in single-cell derived rCHO clones. Simple batch cultures of rCHO cell clones with the highest expression produced 1.0 g/l for aflibercept and 1.4 g/l for pembrolizumab without a time-consuming gene amplification process. Electroporation with DMSO also shortened the development of rCHO cell lines to 2-3 months, allowing rapid establishment of stable rCHO cell lines with a desirable expression level antibodies.
Full-Text
Key_wordDimethyl sulfoxide, stable cell line, chinese hamster ovary cell, recombinant antibody, electroporation
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