2019 ; Vol.29-1: 160~170
|Author||Jing Liu, Guilian yang, Xing gao, Zan zhang, Yang liu, Xin yang, Chunwei shi, Qqiong liu, Yanlong Jiang, Chunfeng wang|
|Title||Immunomodulatory Properties of Lactobacillus plantarum NC8 Expressing an Anti-CD11c Single-Chain Fv Fragment|
J. Microbiol. Biotechnol.2019 ;
|Abstract||The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively
for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host
immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain
with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c).
The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more
efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction
between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with
BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more
efficient expression of GFP compared with wild-type strains shown by flow cytometry
analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs.
Similar results were also observed in an in vivo study, which showed that oral administration
resulted in efficient expression of GFP in both Peyer’s patches (PP) and mesenteric lymph
nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting
strain significantly promoted the differentiation and maturation of DCs, the differentiation of
IL-4+ and IL-17A+ T helper (Th) cells in MLNs, as well as production of B220+ IgA+ B cells in the
PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could
increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a
promising approach for vaccine study.|
|Key_word||Dendritic cell, CD11c, Lactobacillus plantarum, immunomodulatory|
Lechardeur D, Sohn KJ, Haardt M, Joshi PB, Monck M, Graham RW, et al. 1999. Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer. Gene Ther. 6: 482-497.
Vijaya Kumar SG, Singh SK, Goyal P, Dilbaghi N, Mishra DN. 2005. Beneficial effects of probiotics and prebiotics on human health. Die Pharmazie 60: 163-171.
Liu L, Zhang W, Song Y, Wang W, Zhang Y, Wang T, et al. 2018. Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickens. Vaccine. 36: 729-735.
Mancha-Agresti P, de Castro CP, Dos Santos JSC, Araujo MA, Pereira VB, LeBlanc JG, et al. 2017. Recombinant Invasive Lactococcus lactis Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-gamma, IL-6, and TNF-alpha Cytokines after Intranasal Immunization. Front. Microbiol. 8: 1263.
Almeida JF, Breyner NM, Mahi M, Ahmed B, Benbouziane B, Boas PC, et al. 2016. Expression of fibronectin binding protein A (FnBPA) from Staphylococcus aureus at the cell surface of Lactococcus lactis improves its immunomodulatory properties when used as protein delivery vector. Vaccine 34: 1312-1318.
Sun Y, Qian J, Xu X, Tang Y, Xu W, Yang W, et al. 2018. Dendritic cell-targeted recombinant Lactobacilli induce DC activation and elicit specific immune responses against G57 genotype of avian H9N2 influenza virus infection. Vet. Microbiol. 223: 9-20.
Yang WT, Li QY, Ata EB, Jiang YL, Huang HB, Shi CW, et al. 2018. Immune response characterization of mice immunized with Lactobacillus plantarum expressing spike antigen of transmissible gastroenteritis virus. Appl. Microbiol. Biotechnol. 19: 8307-8318.
Rigaux P, Daniel C, Hisbergues M, Muraille E, Hols P, Pot B, et al. 2009. Immunomodulatory properties of Lactobacillus plantarum and its use as a recombinant vaccine against mite allergy. Allergy 64: 406-414.
van Baarlen P, Troost FJ, van Hemert S, van der Meer C, de Vos WM, de Groot PJ, et al. 2009. Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance. Proc. Nat. Aca. Sci. USA 106: 2371-2376.
Figdor CG, van Kooyk Y, Adema GJ. 2002. C-type lectin receptors on dendritic cells and Langerhans cells. Nature Rev. Immunol. 2: 77-84.
Moll H. 2003. Dendritic cells and host resistance to infection. Cell. Microbiol. 5: 493-500.
Bajtay Z, Csomor E, Sandor N, Erdei A. 2006. Expression and role of Fc- and complement-receptors on human dendritic cells. Immunol. lett. 104: 46-52.
Ejaz A, Ammann CG, Werner R, Huber G, Oberhauser V, Horl S, et al. 2012. Targeting viral antigens to CD11c on dendritic cells induces retrovirus-specific T cell responses. PLoS One 7: e45102.
Kanazawa N. 2007. Dendritic cell immunoreceptors: C-type lectin receptors for pattern-recognition and signaling on antigen-presenting cells. J. Dermatol. Sci. 45: 77-86.
Zanoni I, Granucci F. 2010. Regulation of antigen uptake, migration, and lifespan of dendritic cell by Toll-like receptors. J. Mol. Med. 88: 873-880.
Christophe M, Kuczkowska K, Langella P, Eijsink VG, Mathiesen G, Chatel JM. 2015. Surface display of an antiDEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivo. Microb. Cell Fact. 14: 95.
Sorvig E, Mathiesen G, Naterstad K, Eijsink VG, Axelsson L. 2005. High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors. Microbiol. 151: 2439-2449.
Guimaraes V, Innocentin S, Chatel JM, Lefevre F, Langella P, Azevedo V, et al. 2 009. A n ew plasmid v ector f or DNA delivery using lactococci. Genet. Vaccines Ther. 7: 4.
Han X, Wang L, Li W, Li B, Yang Y, Yan H, et al. 2015. Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats. Braz. J. Microbiol. 46:849-854.
Que YA, Haefliger JA, Francioli P, Moreillon P. 2000. Expression of Staphylococcus aureus clumping factor A in Lactococcus lactis subsp. cremoris using a new shuttle vector. Infect. Immun. 68: 3516-3522.
Almeida JF, Mariat D, Azevedo V, Miyoshi A, de Moreno de LeBlanc A, Del Carmen S, et al. 2014. Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo. BMC Microbiol. 14: 248.
Perone MJ, Larregina AT, Shufesky WJ, Papworth GD, Sullivan ML, Zahorchak AF, et al. 2006. Transgenic galectin-1 induces maturation of dendritic cells that elicit contrasting responses in naive and activated T cells. J. Immunol. 176:7207-7220.
Lee S, Miller SA, Wright DW, Rock MT, Crowe JE, Jr. 2007. Tissue-specific regulation of CD8+ T-lymphocyte immunodominance in respiratory syncytial virus infection. J. Virol. 81: 2349-2358.
Mancha-Agresti P, Drumond MM, Carmo FL, Santos MM, Santos JS, Venanzi F, et al. 2017. A new broad range plasmid for DNA delivery in eukaryotic cells using lactic acid bacteria: in vitro and in vivo assays. Mol. Ther. Methods Clin. Dev. 4: 83-91.
Yagnik B, Padh H, Desai P. 2016. Construction of a new shuttle vector for DNA delivery into mammalian cells using non-invasive Lactococcus lactis. Microbes Infect. 18: 237-244.