2019 ; Vol.29-1: 151~159
|Author||JI Eun Ahn, Hangeun Kim, Dae Kyun Chung|
|Place of duty||Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea|
|Title||Lipoteichoic Acid Isolated from Lactobacillus plantarum Maintains Inflammatory Homeostasis through Regulation of Th1- and Th2- Induced Cytokines|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Lipoteichoic acid isolated from Lactobacillus plantarum K8 (pLTA) alleviates lipopolysaccharide
(LPS)-induced excessive inflammation through inhibition of TNF-α and interleukin (IL)-6. In
addition, pLTA increases the survival rate of mice in a septic shock model. In the current
study, we have found that pLTA contributes to homeostasis through regulation of pro- and
anti-inflammatory cytokine production. In detail, pLTA decreased the production of IL-10 by
phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells stimulated with prostaglandin
E2 (PGE-2) and LPS. However, TNF-α production which was inhibited by PGE-2+LPS
increased by pLTA treatment. The regulatory effects of IL-10 and TNF-α induced by PGE-2
and LPS in PMA-differentiated THP-1 cells were mediated by pLTA, but not by other LTAs
isolated from either Staphylococcus aureus (aLTA) or L. sakei (sLTA). Further studies revealed
that pLTA-mediated IL-10 inhibition and TNF-α induction in PGE-2+LPS-stimulated PMAdifferentiated
THP-1 cells were mediated by dephosphorylation of p38 and phosphorylation
of c-Jun N-terminal kinase (JNK), respectively. Reduction of pLTA-mediated IL-10 inhibited
the metastasis of breast cancer cells (MDA-MB-231), which was induced by IL-10 or
conditioned media prepared from PGE-2+LPS-stimulated PMA-differentiated THP-1 cells.
Taken together, our data suggest that pLTA contributes to inflammatory homeostasis through
induction of repressed pro-inflammatory cytokines as well as inhibition of excessive antiinflammatory
|Key_word||Lipoteichoic acid, anti-inflammation, homeostasis, Interleukin-10, tumor necrosis factor-alpha, metastasis of breast cancer|
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