2019 ; Vol.29-1: 30~36
|Author||I Na Yoon, Jae Sam Hwang, Joon Ha Lee, Ho Kim|
|Place of duty||Department of Life Science, College of Natural Science, Daejin University,Pocheon, Gyeonggido, South Korea, 487-711|
|Title||The Antimicrobial Peptide CopA3 Inhibits Clostridium difficile Toxin A-Induced Viability Loss and Apoptosis in Neural Cells|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Numerous studies have reported that enteric neurons involved in controlling
neurotransmitter secretion and motility in the gut critically contribute to the progression of
gut inflammation. Clostridium difficile toxins, which cause severe colonic inflammation, are
also known to affect enteric neurons. Our previous study showed that C. difficile toxin A
directly induces neural cell toxicities, such as viability loss and apoptosis. In the current study,
we attempted to identify a potent inhibitor of toxin A-induced neural cell toxicity that may aid
in managing toxin A-induced gut inflammation. In our recent study, we found that the Korea
dung beetle-derived antimicrobial peptide CopA3 completely blocked neural cell apoptosis
caused by okadaic acid or 6-OHDA. Here, we examined whether the antimicrobial peptide
CopA3 inhibited toxin A-induced neural cell damage. In neuroblastoma SH-SY5Y cells, CopA3
treatment protected against both apoptosis and viability loss caused by toxin A. CopA3 also
completely inhibited activation of the pro-apoptotic factor, caspase-3. Additionally, CopA3
rescued toxin A-induced downregulation of neural cell proliferation. However, CopA3 had no
effect on signaling through ROS/p38 MAPK/p27kip1, suggesting that CopA3 inhibits toxin Ainduced
neural cell toxicity independent of this well-characterized toxin A pathway. Our data
further suggest that ability of CopA3 to rescue toxin A-induced neural cell damage may also
ameliorate the gut inflammation caused by toxin A.|
|Key_word||Bacterial toxin, gut inflammation, enteric nerve system, insect-derived antimicrobial peptide, apoptosis|
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