2019 ; Vol.29-1: 79~90
|Author||Shamsun Nahar, Min-Hye Jeong, Jae-Seoun Hur|
|Place of duty||Department of Environmental Education and Science, Sunchon National University, Suncheon, Republic of Korea,Department of Food Science and Technology, Chung-Ang University, Anseong, Republic of Korea|
|Title||Lichen-Associated Bacterium, a Novel Bioresource of Polyhydroxyalkanoate (PHA) Production and Simultaneous Degradation of Naphthalene and Anthracene|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Lichens are generally known as self-sufficient, symbiotic life-forms between fungi and algae/
cyanobacteria, and they also provide shelter for a wide range of beneficial bacteria. Currently,
bacterial-derived biodegradable polyhydroxyalkanoate (PHA) is grabbing the attention of
many researchers as a promising alternative to non-degradable plastics. This study was
conducted to develop a new method of PHA production using unexplored lichen-associated
bacteria, which can simultaneously degrade two ubiquitous industrial toxins, anthracene and
naphthalene. Here, 49 lichen-associated bacteria were isolated and tested for PHA synthesis.
During the GC-MS analysis, a potential strain of EL19 was found to be a 3-hydroxyhexanoate
(3-HHx) accumulator and identified as Pseudomonas sp. based on the 16S rRNA sequencing.
GC analysis revealed that EL19 was capable of accumulating 30.62% and 19.63% of 3-HHx
from naphthalene and anthracene, respectively, resulting in significant degradation of 98%
and 96% of naphthalene and anthracene, respectively, within seven days. Moreover, the
highly expressed phaC gene verified the genetic basis of PHAmcl production under nitrogen
starvation conditions. Thus, this study strongly supports the hypothesis that lichen-associated
bacteria can detoxify naphthalene and anthracene, store energy for extreme conditions, and
probably help the associated lichen to live in extreme conditions. So far, this is the first
investigation of lichen-associated bacteria that might utilize harmful toxins as feasible
supplements and convert anthracene and naphthalene into eco-friendly 3-HHx. Implementation
of the developed method would reduce the production cost of PHAmcl while removing harmful
waste products from the environment.|
|Key_word||Biodegradation, biopolymers, bioproducts, hazardous waste, Pseudomonas|
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