2019 ; Vol.29-2: 256~267
|Author||Nicola Di Fidio, Federico Liuzzi, Silvio Mastrolitti, Roberto Albergo, Isabella De Bari|
|Place of duty||University of Pisa, Italy.|
|Title||Single Cell Oil Production from Undetoxified Arundo donax L. hydrolysate by Cutaneotrichosporon curvatus|
J. Microbiol. Biotechnol.2019 ;
|Abstract||The use of low-cost substrates represents one key issue to make single cell oil production
sustainable. Among low-input crops, Arundo donax L. is a perennial herbaceous rhizomatous
grass containing both C5 and C6 carbohydrates. The scope of the present work was to
investigate and optimize the production of lipids by the oleaginous yeast Cutaneotrichosporon
curvatus from undetoxified lignocellulosic hydrolysates of steam-pretreated A. donax. The
growth of C. curvatus was first optimized in synthetic media, similar in terms of sugar
concentration to hydrolysates, by applying the response surface methodology (RSM) analysis.
Then the bioconversion of undetoxified hydrolysates was investigated. A fed-batch process for
the fermentation of A. donax hydrolysates was finally implemented in a 2-L bioreactor. Under
optimized conditions, the total lipid content was 64% of the dry cell weight and the lipid yield
was 63% of the theoretical. The fatty acid profile of C. curvatus triglycerides contained 27%
palmitic acid, 33% oleic acid and 32% linoleic acid. These results proved the potential of lipid
production from A. donax, which is particularly important for their consideration as
substitutes for vegetable oils in many applications such as biodiesel or bioplastics.|
|Key_word||Arundo donax L. hydrolysates, cutaneotrichosporon curvatus, single cell oil, second generation sugars, microbial lipids|
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