2018 ; Vol.28-10: 1626~1634
|Author||Seon-Weon Hwang, Xiao Sun, Jun-Hyuk Han, Tae-Yeon Kim, Sushruta Koppula, Tae-Bong Kang, Jae-Kwan Hwang, Kwang Ho Lee|
|Place of duty||Graduate Program in Biomaterials Science and Engineering, Yonsei University, Seoul 03722, Republic of Korea|
|Title||Fermentation-Mediated Enhancement of Ginseng’s Anti-Allergic Activity against IgE-Mediated Passive Cutaneous Anaphylaxis In Vivo and In Vitro|
J. Microbiol. Biotechnol.2018 ;
|Abstract||Ginseng (the root of Panax ginseng Meyer) fermented by Lactobacillus plantarum has been found
to attenuate allergic responses in in vitro and in vivo experimental models. Ginseng has been
reported to also possess various biological functions including anti-inflammatory activity. The
present study was aimed at comparing the anti-allergic effect of ginseng and fermented
ginseng extracts on IgE-mediated passive cutaneous anaphylaxis in vitro in a murine cell line
and in vivo in mice. Fermented ginseng extract (FPG) showed higher inhibitory effect against
in vitro and in vivo allergic responses when compared with ginseng extract (PG). The
secretion of β-hexosaminidase and interleukin (IL)-4 from the IgE-DNP-stimulated RBH-2H3
mast cells were significantly (p < 0.05) inhibited by FPG treatment, and this effect was
concentration-dependent. Further, MKK4 activation and subsequent JNK phosphorylation
were attenuated by FPG treatment. The inhibitory effect of FPG on the in vitro allergic
response was verified in vivo against IgE-DNP–induced passive cutaneous anaphylaxis in a
mouse model. These data indicated that the fermentation of ginseng with L. plantarum
enhanced its anti-allergic effects both in vitro and in vivo. We predict that compositional
changes in the ginsenosides caused by the fermentation may contribute to the change in the
anti-allergic effects of ginseng. The results of our study highlight the potential of the use of
FPG as a potential anti-allergic agent.|
|Key_word||Ginseng, fermentation, anti-allergic, anaphylaxis, Lactobacillus plantarum|
Jeon J, Kim Y, Kim H, Kang JS. Lee WJ. 2015. Antiinflammatory effect of alloferon on ovalbumin-induced asthma. Immune Netw. 15: 304-312.
Han SY, Bae JY, Park SH, Kim YH, Park JH, Kang YH. 2013. Resveratrol inhibits IgE-mediated basophilic mast cell degranulation and passive cutaneous anaphylaxis in mice. J. Nutr. 143: 632-639.
Wang L, Zhang F, Cao Z, Xiao Y, Li S, Yu B, Qi J. 2017. Ginsenoside F2 induces the release of mediators associated with Anaphylactoid reactions. Fitoterapia 121: 223-228.
Lee JH, Kim JW, Kim DJ, Kim HS, Park HJ, Park DK, et al. 2011. The Src family kinase Fgr is critical for activation of mast cells and IgE-mediated anaphylaxis in mice. J. Immunol. 187: 1807-1815.
Lim SJ, Kim M, Randy A, Nho CW. 2015. Inhibitory effect of the branches of Hovenia dulcis Thunb. and its constituent pinosylvin on the activities of IgE-mediated mast cells and passive cutaneous anaphylaxis in mice. Food Funct. 6: 13611370.
Je IG, Kim DS, Kim SW, Lee S, Lee HS, Park EK, et al. 2015. Tyrosol suppresses allergic inflammation by inhibiting the activation of phosphoinositide 3-kinase in mast cells. PLoS One 10: e0129829.
Park KI, Kim DG, Yoo JM, Ma JY. 2016. The Herbal Medicine KIOM-MA128 inhibits the antigen/ige-mediated allergic response in vitro and in vivo. Molecules 21: E1015.
Turner H, Kinet JP. 1999. Signalling through the highaffinity IgE receptor FcεRI. Nature 402: B 24-30.
Kawakami Y, Hartman SE, Holland PM, Cooper JA, Kawakami T. 1998. Multiple signaling pathways for the activation of JNK in mast cells: involvement of Bruton's tyrosine kinase, protein kinase C, and JNK kinases, SEK1 and MKK7. J. Immunol. 161: 1795-1802.
Song JS, Haleem-Smith H, Arudchandran R, Gomez J, Scott PM, Mill JF, et al. 1999. Tyrosine phosphorylation of Vav stimulates IL-6 production in mast cells by a Rac/c-Jun Nterminal kinase-dependent pathway. J. Immunol. 163: 802-810.
Garrington TP, Ishizuka T, Papst PJ, Chayama K, Webb S, Yujiri T, et al. 2000. MEKK2 gene disruption causes loss of cytokine production in response to IgE and c-Kit ligand stimulation of ES cell-derived mast cells. EMBO J. 19: 53875395.
Trinh H, Ba EA, Hyun YJ, Jang YA, Yun HK, Hong SS, Kim DH. 2010. Anti-allergic effects of fermented Ixeris sonchifolia and its constituent in mice. J. Microbiol Biotechnol. 20: 217-223.
Baeg IH, So SH. 2013. The world ginseng market and the ginseng (Korea). J. Ginseng Res. 37: 1-7.
Jiao L, Li B, Wang M, Liu Z, Zhang X, Liu S. 2014. Antioxidant activities of the oligosaccharides from the roots, flowers and leaves of Panax ginseng C.A. Meyer. Carbohydr. Polym. 106: 293-298.
Kim DY, Ro JY, Lee CH. 2015. 20 (S)-Protopanaxatriol inhibits release of inflammatory mediators in immunoglobulin Emediated mast cell activation. J. Ginseng Res. 39: 189-198.
Lim CY, Moon JM, Kim BY, Lim SH, Lee GS, Yu HS, et al. 2015. Comparative study of Korean white ginseng and Korean red ginseng on efficacies of OVA-induced asthma model in mice. J. Ginseng Res. 39: 38-45.
Liu S, Han Ye, Zhou Z. 2011. Lactic acid bacteria in traditional fermented Chinese foods. Food Res. Int. 44: 643-651.
Vizoso Pinto MG , Franz CM, Schillinger U, Holzapfel WH. 2006. Lactobacillus spp. with in vitro probiotic properties from human faeces and traditional fermented products. Int. J. Food Microbiol. 109: 205-214.
Jung JH, Kang IG, Kim DY, Hwang YJ, Kim ST. 2013. The effect of Korean red ginseng on allergic inflammation in a murine model of allergic rhinitis. J. Ginseng Res. 37: 167-175.
Lee EJ, Song MJ, Kwon HS, Ji GE, Sung MK. 2012. Oral administration of fermented red ginseng suppressed ovalbumininduced allergic responses in female BALB/c mice. Phytomedicine 19: 896-903.
Ribeiro-Filho J, Leite FC, Costa HF, Calheiros AS, Torres RC, de Azevedo CT, et al. 2014. Curine inhibits mast celldependent responses in mice. J. Ethnopharmacol. 155: 11181124.
Park J, Seo JS, Kim SA, Shin S, Park JY, Han NS. 2017. Microbial diversity of commercial makgeolli and its influence on the organoleptic characteristics of Korean rice sourdough, jeung-pyun. J. Microbiol Biotechnol. 27: 1736-1743.
Suzuki K, Verma IM. 2008. Phosphorylation of SNAP-23 by IκB kinase 2 regulates mast cell degranulation. Cell 134: 485495.
Shim DW, Han JW, Sun X, Jang CH, Koppula S, Kim TJ, Kang TB, Lee KH. 2013. Lysimachia clethroides Duby extract attenuates inflammatory response in Raw 264.7 macrophages stimulated with lipopolysaccharide and in acute lung injury mouse model. J. Ethnopharmacol. 150: 10071015.
Bischoff SC. 2007. Role of mast cells in allergic and nonallergic immune responses: comparison of human and murine data. Nat. Rev. Immunol. 7: 93-104.
Lian Q, Cheng Y, Zhong C, Wang F. 2015. Inhibition of the IgE-mediated activation of RBL-2H3 cells by TIPP, a novel thymic immunosuppressive pentapeptide. Int. J. Mol. Sci. 16: 2252-2268.
Choi P, Reiser H. 1998. IL-4: role in disease and regulation of production. Clin. Exp. Immunol. 113: 317-319.
Lee J. 2016. Regulation of IgE-mediated food allergy by IL-9 producing mucosal mast cells and type 2 innate lymphoid cells. Immune Netw. 16: 211-218.
Xiao S, Jiang J, Shim D, Kim T, Kang T, Lee K. 2014. Antiallergic effect of ethanolic extract of Flos Sophora japonica L. on Ca ionophore stimulated murine RBL-2H3 cells. J. Korean Soc. Food Sci. Nutr. 43: 349-354.
Burton OT, Oettgen HC. 2011. Beyond immediate hypersensitivity:evolving roles for IgE antibodies in immune homeostasis and allergic diseases. Immunol. Rev. 242: 128-143.
Tournier C, Dong C, Turner TK, Jones SN, Flavell RA, Davis RJ. 2001. MKK7 is an essential component of the JNK signal transduction pathway activated by proinflammatory cytokines. Genes Dev. 15: 1419-1426.
Zheng H, Jeong Y , Song J , Ji G E. 2 011. O ral administration of ginsenoside Rh1 inhibits the development of atopic dermatitis-like skin lesions induced by oxazolone in hairless mice. Int. Immunopharmacol. 11: 511-518.
Li LC, Piao HM, Z heng M Y, L in ZH, C hoi YH, Yan G H. 2015. Ginsenoside Rh2 attenuates allergic airway inflammation by modulating nuclear factor-κB activation in a murine model of asthma. Mol. Med. Rep. 12: 6946-6954.
Sun J, Song X, Hu S. 2008. Ginsenoside Rg1 and aluminum hydroxide synergistically promote immune responses to ovalbumin in BALB/c mice. Clin. Vaccine Immunol. 15: 303-307.
Lee IS, Uh I, Kim KS, Kim KH, Park J, Kim Y, et al. 2016. Anti-inflammatory effects of ginsenoside Rg3 via NF-κB pathway in A549 cells and human asthmatic lung tissue. J. Immunol. Res. 2016: 1-11.
Oh HA, Seo JY, Jeong HJ, Kim HM. 2013. Ginsenoside Rg1 inhibits the TSLP production in allergic rhinitis mice. Immunopharmacol. Immunotoxicol. 35: 678-686.