2019 ; Vol.29-1: 141~150
|Author||Li Yun Qing, Xue Qing Jie, Yang Yuan Yuan, Wang Hui, Li Xiu Zhen|
|Title||A Role of YlBud8 in the Regulation of Cell Separation in the Yeast Yarrowia lipolytica|
J. Microbiol. Biotechnol.2019 ;
|Abstract||The spatial landmark protein Bud8 plays a crucial role in bipolar budding in the budding
yeast Saccharomyces cerevisiae. The unconventional yeast Yarrowia lipolytica can also bud in a
bipolar pattern, but is evolutionarily distant from S. cerevisiae. It encodes the protein
YALI0F12738p, which shares the highest amino acid sequence homology with S. cerevisiae
Bud8, sharing a conserved transmembrane domain at the C-terminus. Therefore, we named it
YlBud8. Deletion of YlBud8 in Y. lipolytica causes cellular separation defects, resulting in
budded cells remaining linked with one another as cell chains or multiple buds from a single
cell, which suggests that YlBud8 may play an important role in cell separation, which is
distinct from the function of Bud8 in S. cerevisiae. We also show that the YlBud8-GFP fusion
protein is located at the cell membrane and enriched in the bud cortex, which would be
consistent with a role in the regulation of cell separation. The coiled-coil domain at the Nterminus
of YlBud8 is important to the correct localization and function of YlBud8, as
truncated proteins that do not contain the coiled-coil domain cannot rescue the defects
observed in Ylbud8Δ. This finding suggests that a new signaling pathway controlled by
YlBud8 via regulation of cell separation may exist in Y. lipolytica.|
|Key_word||YlBud8, cell polarity, cell separation, coiled-coil|
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