2019 ; Vol.29-9: 1478~1487
|Author||Weiwei Wang, Wentao Xing, Sichen Wei, Qiaoying Gao, Xinliang Wei, Liang Shi, Yu Kong, Zhenhua Su|
|Place of duty||Department of Gastroenterology, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou, Hebei 061001, P.R. China|
|Title||Semi-Rational Screening of Probiotics from the Fecal Flora of Healthy Adults against DSS-Induced Colitis Mice by Enhancing Anti-Inflammatory Activity and Modulating the Gut Microbiota|
J. Microbiol. Biotechnol.2019 ;
|Abstract||Ulcerative colitis (UC), a chronic inflammatory bowel disease, substantially impacts patients’
health-related quality of life. In this study, an effective strategy for discovering high-efficiency
probiotics has been developed. First, in order to survive in the conditions of the stomach and
intestine, high bile salt-resistant and strong acid-resistant strains were screened out from the
fecal flora of healthy adults. Next, the probiotic candidates were rescreened by examining the
induction ability of IL-10 (anti-inflammatory factor) production in dextran sodium sulfate
(DSS)-induced colitis mice, and Lactobacillus sakei 07 (L07) was identified and selected as
probiotic P. In the end, fourteen bifidobacterium strains isolated from stools of healthy males
were examined for their antimicrobial activity. Bifidobacterium bifidum B10 (73.75% inhibition
rate) was selected as probiotic B. Moreover, the colonic IL-6 and TNF-α expression of the DSSinduced
colitis mice treated with L. sakei 07 (L07) – B. bifidum B10 combination (PB)
significantly decreased and the IL-10 expression was up-regulated by PB compared to the DSS
group. Furthermore, Bacteroidetes and Actinobacteria decreased and Firmicutes increased in the
DSS group mice, significantly. More interestingly, the intestinal flora biodiversity of DSS
colitis mice was increased by PB. Of those, the level of B. bifidum increased significantly. The
Bacteriodetes/Firmicutes (B/F) ratio increased, and the concentration of homocysteine and LPS
in plasma was down-regulated by PB in the DSS-induced colitis mice. Upon administration of
PB, the intestinal permeability of the the DSS-induced colitis mice was decreased by
approximately 2.01-fold. This method is expected to be used in high-throughput screening of
the probiotics against colitis. In addition, the L. sakei 07 – B. bifidum B10 combination holds
potential in UC remission by immunomodulatory and gut microbiota modulation|
|Key_word||Probiotics, ulcerative colitis, anti-inflammatory, gut microbiota, immunomodulatory, ntestinal permeability|
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