2018 ; Vol.28-5: 718~731
|Author||Soyoung Yeo, Hee Sung Shin, Hye Won Lee, Doseon Hong, Hyunjoon Park, Wilhelm Holzapfel, Eun Bae Kim, Chul Sung Huh|
|Place of duty||Research Institute of Eco-friendly Livestock Science, Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon-do, South Korea|
|Title||Determination of Optimized Growth Medium and Cryoprotective Additives to Enhance the Growth and Survival of Lactobacillus salivarius|
J. Microbiol. Biotechnol.2018 ;
|Abstract||The beneficial effects of lactic acid bacteria (LAB) have been intensively investigated in recent
decades with special focus on modulation of the host intestinal microbiota. Numerous
discoveries of effective probiotics are driven by a significantly increasing demand for dietary
supplements. Consequently, technological advances in the large-scale production and
lyophilization are needed by probiotic-related industries for producing probiotic LAB for
commercial use. Our study had a dual objective, to determine the optimum growth medium
composition and to investigate appropriate cryoprotective additives (CPAs) for Lactobacillus
salivarius, and compare its responses with other Lactobacillus species. The one-factor-at-a-time
method and central composite design were applied to determine the optimal medium
composition for L. salivarius cultivation. The following composition of the medium was
established (per liter): 21.64 g maltose, 85 g yeast extract, 1.21 ml Tween 80, 6 g sodium acetate,
0.2 g MgSO4∙7H2O, 0.02 g MnSO4∙H2O, 1 g K2HPO4, 1.5 g KH2PO4, 0.01 g FeSO4∙7H2O, and 1 g
sodium citrate. A cryoprotective additive combination comprising 10% (w/v) skim milk and
10% (w/v) sucrose supplemented with 2.5% (w/v) sodium glutamate was selected for
L. salivarius, and its effectiveness was confirmed using culture-independent methods in the
freeze-dried cells of the Lactobacillus strains. In conclusion, the optimized medium enhanced
the species-specific cultivation of L. salivarius. On the other hand, the cryoprotective effects of
the selected CPA mixture may also be dependent on the bacterial strain. This study highlights
the necessity for precise and advanced processing techniques for large-scale production of
probiotics in the food and feed industries.|
|Key_word||Lactic acid bacteria, Cultivation, Growth medium, Freeze-drying, Viability, Culture-independent method|
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