Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2018 ; Vol.28-8: 1384~1390

AuthorFeng Liu, Yue-ping Cao
Place of dutyPlant Science Department, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R. China
TitleImprovement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Pantoea sp. 5-Enolpyruvylshikimate-3- Phosphate Synthase
PublicationInfo J. Microbiol. Biotechnol.2018 ; Vol.28-8
AbstractGlyphosate inhibits the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway. A mutant of EPSPS from Pantoea sp. was identified using site-directed mutagenesis (SDM). The mutant significantly improved glyphosate resistance. The mutant had mutations in three amino acids: Gly97 to Ala, Thr 98 to Ile and Pro 102 to Ser. These mutation sites in E.coli have been studied as significant active sites of glyphosate resistance. However, in our research they were found to jointly contribute to the improvement of glyphosate tolerance. In addition, the level of glyphosate tolerance in transgenic Arabidopsis confirmed the potentiality of mutant in breeding glyphosate-resistant plants.
Full-Text
Key_word5-Enopyruvylshikimate-3-phosphate synthase, Pantoea sp., glyphosate, mutant, transgenic Arabidopsis
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