2018 ; Vol.28-3: 381~390
|Author||Ji-Sun Kim, Ji-Ho Jeong, Yongae Kim|
|Place of duty||Department of Chemistry, Hankuk University of Foreign Studies, Yong-In 17035, Republic of Korea|
|Title||Design and Engineering of Antimicrobial Peptides Based on LPcin-YK3, an Antimicrobial Peptide Derivative from Bovine Milk|
J. Microbiol. Biotechnol.2018 ;
|Abstract||We have previously derived a novel antimicrobial peptide, LPcin-YK3(YK3), based on
lactophoricin and have successfully studied and reported on the relationship between its
structure and function. In this study, antimicrobial peptides with improved antimicrobial
activity, less cytotoxicity, and shorter length were devised and characterized on the basis of
YK3, and named YK5, YK8, and YK11. The peptide design was based on a variety of
knowledge, and a total of nine analog peptides consisted of one to three amino acid
substitutions and C-terminal deletions. In detail, tryptophan substitution improved the
membrane perturbation, lysine substitution increased the net charge, and excessive
amphipathicity decreased. The analog peptides were examined for structural characteristics
through spectroscopic analytical techniques, and antimicrobial susceptibility tests were used
to confirm their activity and safety. We expect that these studies will provide a platform for
systematic engineering of new antibiotic peptides and generate libraries of various antibiotic
|Key_word||Antimicrobial peptide, lactophoricin, modification, net positive charge, tryptophan, amphipathicity|
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