2018 ; Vol.28-3: 375~380
|Author||Hee-Young Ahn, Young-Moo Choo, Young-Su Cho|
|Place of duty||Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 49315, Republic of Korea|
|Title||Anti-Pigmentation Effects of Eight Phellinus linteus-Fermented Traditional Crude Herbal Extracts on Brown Guinea Pigs of Ultraviolet B-Induced Hyperpigmentation|
J. Microbiol. Biotechnol.2018 ;
|Abstract||We have previously found that mycelia culture broth of eight kinds of traditional herbal
extracts fermented with Phellinus linteus (previously named as 8-HsPLCB) not only inhibited
melanin and tyrosinase activity, but also reduced the contents of melanogenesis-related
proteins, including tyrosinase and microphthalmia-associated transcription factor, in 3-
isobutyl-1-methylxanthine-stimulated B16F0 melanoma cells. For a further study, the effect of
8-HsPLCB against skin pigmentation in brown guinea pigs with ultraviolet B (UVB)-induced
hyperpigmentation was investigated. 8-HsPLCB (3%) and arbutin (2%) as positive controls
were applied topically twice daily for 4 weeks to the hyperpigmented areas. 8-HsPLCB
showed skin-lightening effect as effective as arbutin, one of the most widely used in whitening
cosmetics. Melanin index values as the degree of pigmentation showed a significant reduction
week by week post 8-HsPLCB treatment and then substantially reduced by 4 weeks. The
degree of depigmentation after 4 weeks of topical application with 8-HsPLCB was 32.2% as
compared with before treatment (0 week). Moreover, using Fontana-Masson staining and
hematoxylin-eosin staining, 8-HsPLCB reduced melanin pigmentation in the basal layer of the
epidermis and epidermal thickness changes exposed to the UV-B irradiation as compared with
non-treatment and vehicle treatment. The intensity of the skin-lightening effect of 8-HsPLCB
was similar to arbutin. These results suggest that the skin-lightening effect of 8-HsPLCB might
be resulted from inhibition of melanin synthesis by tyrosinase in melanocytes. To conclude,
8-HsPLCB treatment showed reduction of the melanin pigment and histological changes
induced by UV irradiation in brown guinea pigs.|
|Key_word||Fermentation, melanin index, skin-whitening effect, UV radiation, epidermal thickness|
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