2018 ; Vol.28-1: 40~49
|Author||Seon Kyeong Park, Jin Yong Kang, Jong Min Kim, Sang Hyun Park, Bong Seok Kwon, Gun-Hee Kim, Ho Jin Heo|
|Place of duty||Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea|
|Title||Protective Effect of Fucoidan Extract from Ecklonia cava on Hydrogen Peroxide-Induced Neurotoxicity|
J. Microbiol. Biotechnol.2018 ;
|Abstract||We evaluated the antioxidant activity and neuronal cell-protective effect of fucoidan extract
from Ecklonia cava (FEC) on hydrogen peroxide (H2O2)-induced cytotoxicity in PC-12 and MCIXC
cells to assess its protective effect against oxidative stress. Antioxidant activities were
examined using the ABTS radical scavenging activity and malondialdehyde-inhibitory effect,
and the results showed that FEC had significant antioxidant activity. Intracellular ROS contents
and neuronal cell viability were investigated using the DCF-DA assay and MTT reduction
assay. FEC also showed remarkable neuronal cell-protective effect compared with vitamin C
as a positive control for both H2O2-treated PC-12 and MC-IXC cells. Based on the neuronal cellprotective
effects, mitochondrial function was analyzed in PC-12 cells, and FEC significantly
restored mitochondrial damage by increasing the mitochondrial membrane potential (Δψm)
and ATP levels and regulating mitochondrial-mediated proteins (p-AMPK and BAX). Finally,
the inhibitory effects against acetylcholinesterase (AChE), which is a critical hydrolyzing
enzyme of the neurotransmitter acetylcholine in the cholinergic system, were investigated (IC50
value = 1.3 mg/ml) and showed a mixed (competitive and noncompetitive) pattern of inhibition.
Our findings suggest that FEC may be used as a potential material for alleviating oxidative
stress-induced neuronal damage by regulating mitochondrial function and AChE inhibition.|
|Key_word||Ecklonia cava, fucoidan, oxidative stress, neuronal cells, mitochondria|
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