2017 ; Vol.27-12: 2180~2189
|Author||Hao Tan, Jie Tang, Xiaolin Li, Tianhai Liu, Renyun Miao, Zhongqian Huang, Yong Wang, Bingcheng Gan, Weihong Peng|
|Place of duty||National-Local Joint Engineering Laboratory of Breeding and Cultivation of Edible and Medicinal Fungi, Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R. China,Scientific Observing and Experimental Station of Agro-microbial Resource and Utilization in Southwest China, Ministry of Agriculture, Chengdu 610066, P.R. China|
|Title||Biochemical Characterization of a Psychrophilic Phytase from an Artificially Cultivable Morel Morchella importuna|
J. Microbiol. Biotechnol.2017 ;
|Abstract||Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of the histidine
acid phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic
mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest
hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified, and characterized.
The phytase had an optimum temperature at 25°C, which is the lowest among all the known
phytases to our best knowledge. The optimum pH (6.5) is higher than most of the known HAP
phytases, which is fit for the weak acidic condition in fish gut. At the optimum pH and
temperature, MiPhyA showed the maximum activity level (2,384.6 ± 90.4 μmol·min-1·mg-1,
suggesting that the enzyme possesses a higher activity level over many known phytases at low
temperatures. The phytate-degrading efficacy was tested on three common feed materials
(soybean meal/rapeseed meal/corn meal) and was compared with the well-known phytases
of Escherichia coli and A. niger. When using the same amount of activity units, MiPhyA could
yield at least 3× more inorganic phosphate than the two reference phytases. When using the
same weight of protein, MiPhyA could yield at least 5× more inorganic phosphate than the
other two. Since it could degrade phytate in feed materials efficiently under low temperature
and weak acidic conditions, which are common for aquacultural application, MiPhyA might
be a promising candidate as a feed additive enzyme.|
|Key_word||Morchella importuna, phytase, psychrophilic, optimum at weak acidic pH, phosphate yield from feed material|
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