Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2017 ; Vol.27-11: 1961~1970

AuthorJoon Seob Seong, Song Hua Xuan, So Hyun Park, Keon Soo Lee, Young Min Park, Soo Nam Park
Place of dutyDepartment of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea
TitleAntioxidative and Antiaging Activities and Component Analysis of Lespedeza cuneata G. Don Extracts Fermented with Lactobacillus pentosus
PublicationInfo J. Microbiol. Biotechnol.2017 ; Vol.27-11
AbstractLespedeza cuneata G. Don is a traditional herb that has been associated with multiple biological activities. In this study, we investigated the antioxidative/antiaging activities and performed an active component analysis of the non-fermented and fermented (using Lactobacillus pentosus) extracts of Lespedeza cuneata G. Don. The antioxidative activities of the fermented extract were higher than those of non-fermented extracts. The elastase inhibitory activity, inhibitory effects on UV-induced MMP-1 expression, and ability to promote type I procollagen synthesis were investigated in Hs68 human fibroblasts cells. These tests also revealed that the fermented extract had increased antiaging activities compared with the non-fermented extract. A component analysis of the ethyl acetate fractions of non-fermented and fermented extracts was performed using TLC, HPLC, and LC/ESI-MS/MS to observe changes in the components before and after fermentation. Six components that were different before and after fermentation were investigated. It was thought that kaempferol and quercetin were converted from kaempferol glucosides and quercetin glucosides, respectively, via bioconversion with the fermentation strain. These results indicate that the fermented extract of L. cuneata G. Don has potential for use as a natural cosmetic material with antioxidative and antiaging effects.
Full-Text
Supplemental Data
Key_wordLespedeza cuneata G. Don, Lactobacillus pentosus, fermentation, antioxidative activity, antiaging activity
References
  1. Huynh TN, Smagghe G, Gonzales GB, Camp JV, Raes K. 2016. Extraction and bioconversion of kaempferol metabolites from cauliflower outer leaves through fungal fermentation. Biochem. Eng. J. 116: 27-33.
    CrossRef
  2. Wang L, Wei W, Tian X, Shi K, Wu Z. 2016. Improving bioactivities of polyphenol extracts from Psidium guajava L. leaves through co-fermentation of Monascus anka GIM 3.592 and Saccharomyces cerevisiae GIM 2.139. Ind. Crops Prod. 94: 206-215.
    CrossRef
  3. Lee NK, Paik HD. 2017. Bioconversion using lactic acid bacteria: ginsenosides, GABA, and phenolic compounds. J. Microbiol. Biotechnol. 27: 869-877.
    Pubmed CrossRef
  4. Okabe Y, Shimazu T, Tanimoto H. 2011. Higher bioavailability of isoflavones after a single ingestion of aglycone-rich fermented soybeans compared with glucoside-rich nonfermented soybeans in Japanese postmenopausal women. J. Sci. Food Agric. 91: 658-663.
    Pubmed CrossRef
  5. Schmidt CG, Goncalves LM, Prietto L, Hackbart HS, Furlong EB. 2014. Antioxidant activity and enzyme inhibition of phenolic acids from fermented rice bran with fungus Rhizopus oryzae. Food Chem. 146: 371-377.
    Pubmed CrossRef
  6. Park BG, Jung HJ, Cho YW, Lim HW, Lim CJ. 2013. Potentiation of antioxidative and anti-inflammatory properties of cultured wild ginseng root extract through probiotic fermentation. J. Pharm. Pharmacol. 65: 457-464.
    Pubmed CrossRef
  7. Yang HJ, Kim EH, Park JO, Kim JE, Park SN. 2009. Antioxidative activity and component analysis of fermented Melissa officinalis extracts. J. Soc. Cosmet. Sci. Korea 35: 47-55.
  8. Ahn YJ, Won BR, Kang MK, Kim JH, Park SN. 2009. Antioxidant activity and component analysis of fermented Lavandula angustifolia extracts. J. Soc. Cosmet. Sci. Korea 35: 125-134.
  9. Im AR, Song JH, Lee MY, Yeon SH, Um KA, Chae S. 2014. Anti-wrinkle effects of fermented and non-fermented Cyclopia intermedia in hairless mice. BMC Complement. Altern. Med. 14: 424-429.
    Pubmed CrossRef Pubmed Central
  10. Tsai CC, Chan CF, Huang WY, Lin JS, Chan P, Liu HY, et al. 2013. Applications of Lactobacillus rhamnosus spent culture supernatant in cosmetic antioxidation, whitening and moisture retention applications. Molecules 18: 14161-14171.
    Pubmed CrossRef
  11. Kammeyer A, Luiten RM. 2015. Oxidation events and skin aging. Ageing Res. Rev. 21: 16-29.
    Pubmed CrossRef
  12. Kim M, Park YG, Lee HJ, Lim SJ, Nho CW. 2015. Youngiasides A and C isolated from Youngia denticulatum inhibit UVBinduced MMP expression and promote type I procollagen production via repression of MAPK/AP-1/NF-κB and activation of AMPK/Nrf2 in HaCaT cells and human dermal fibroblasts. J. Agric. Food Chem. 63: 5428-5438.
    Pubmed CrossRef
  13. Kim YH, Ryu SN. 2008. Antioxidant activity of methanol extract from aerial parts in Lespedeza cuneata G. Don. Korean J. Crop Sci. 53: 121-123.
  14. Kwon DJ, Kim JK, Ham YH, Bae YS. 2007. Flavone glycosides from the aerial parts of Lespedeza cuneata G. Don. J. Korean Soc. Appl. Biol. Chem. 50: 344-347.
  15. Lee HJ, Lim GN, Park MA, Park SN. 2011. Antibacterial and antioxidative activity of Lespedeza cuneata G. Don extracts. Korean J. Microbiol. Biotechnol. 39: 63-69.
  16. Ridwan BU, Koning CJ, Besselink MG, Timmerman HM, Brouwer EC, Verhoef J, et al. 2008. Antimicrobial activity of a multispecies probiotic (Ecologic 641) against pathogens isolated from infected pancreatic necrosis. Lett. Appl. Microbiol. 46: 61-67.
    Pubmed
  17. Quan LH, Kim YJ, Li GH, Choi KT, Yang DC. 2013. Microbial transformation of ginsenoside Rb1 to compound K by Lactobacillus paralimentarius. World J. Microbiol. Biotechnol. 29: 1001-1007.
    Pubmed CrossRef
  18. Pyo YH, Lee TC, Lee YC. 2005. Enrichment of bioactive isoflavones in soymilk fermented with β-glucosidase-producing lactic acid bacteria. Food Res. Int. 38: 551-559.
    CrossRef
  19. Min JW, Kim HJ, Joo KS, Kang HC. 2015. Isolation of Stenotrophomonas rhizopilae strain GFC09 with ginsenoside converting activity and anti-wrinkle effects of converted ginsenosides. J. Soc. Cosmet. Sci. Korea 41: 375-382.
    CrossRef
  20. Kim SH, Min JW, Quan LH, Lee S, Yang DU, Yang DC. 2012. Enzymatic transformation of ginsenoside Rb1 by Lactobacillus pentosus strain 6105 from kimchi. J. Ginseng Res. 36: 291-297.
    Pubmed CrossRef Pubmed Central
  21. Park SN, Won DH, Hwang JP, Han SB. 2014. Cellular protective effects of dehydroeffusol isolated from Juncus effusus L. and the mechanisms underlying these effects. J. Ind. Eng. Chem. 20: 3046-3052.
    CrossRef
  22. Tsuji N, Moriwaki S, Suzuki Y, Takema Y, Imokawa G. 2001. The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem. Photobiol. 74: 283-290.
    CrossRef
  23. Tsukahara K, Takema Y, Moriwaki S, Tsuji N, Suzuki Y, Fujimura T, et al. 2001. Selective inhibition of skin fibroblast elastase elicits a concentration-dependent prevention of ultraviolet B-induced wrinkle formation. J. Invest. Dermatol. 117: 671-677.
    Pubmed CrossRef
  24. Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M. 2016. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int. J. Mol. Sci. 17: 868-887.
    Pubmed CrossRef Pubmed Central
  25. Waridel P, Wolfender JL, Ndjoko K, Hobby KR, Major HJ, Hostettmann K. 2001. Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers. J. Chromatogr. A 926: 29-41.
    CrossRef
  26. Sánchez-Rabaneda F, Jáuregui O, Lamuela-Raventós RM, Bastida J, Viladomat F, Codina C. 2003. Identification of phenolic compounds in artichoke waste by high-performance liquid chromatography–tandem mass spectrometry. J. Chromatogr. A 1008: 57-72.
    CrossRef
  27. Ren Q, Wu C, Ren Y, Zhang J. 2013. Characterization and identification of the chemical constituents from tartary buckwheat (Fagopyrum tataricum Gaertn) by high performance liquid chromatography/photodiode array detector/linear ion trap FTICR hybrid mass spectrometry. Food Chem. 136: 1377-1389.
    Pubmed CrossRef
  28. Kim JE, Kim WY, Kim JW, Park HS, Lee SH, Lee SY, et al. 2010. Antibacterial, antioxidative activity and component analysis of Pinus koraiensis leaf extracts. J. Soc. Cosmet. Sci. Korea 36: 303-314.
  29. De Leo M, Abreu MBD, Pawlowska AM, Cioni PL, Braca A. 2010. Profiling the chemical content of Opuntia ficus-indica flowers by HPLC–PDA-ESI-MS and GC/EIMS analyses. Phytochem. Lett. 3: 48-52.
    CrossRef
  30. Yoo G, Park SJ, Lee TH, Yang H, Baek YS, Kim N, et al. 2015. Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated BV-2 microglia cells. Pharmacogn. Mag. 11: 651-656.
    Pubmed CrossRef Pubmed Central
  31. Jeon SM, Kim SI, Ahn JY, Park SN. 2007. Antioxidative properties of extract/fractions of Suaeda asparagoides and Salicornia herbacea extracts (I). J. Soc. Cosmet. Sci. Korea 33: 145-152.
  32. Kim DH, Kim JH, Baek SH, Seo JH, Kho YH, Oh TK, et al. 2004. Enhancement of tyrosinase inhibition of the extract of Veratrum patulum using cellulase. Biotechnol. Bioeng. 87: 849-854.
    Pubmed CrossRef
  33. Chiang HM, Chen HC, Lin TJ, Shih IC, Wen KC. 2012. Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts. Food Chem. Toxicol. 50: 4260-4269.
    Pubmed CrossRef



Copyright © 2009 by the Korean Society for Microbiology and Biotechnology.
All right reserved. Mail to jmb@jmb.or.kr
Online ISSN: 1738-8872    Print ISSN: 1017-7825    Powered by INFOrang.co., Ltd