2017 ; Vol.27-4: 701~708
|Author||Minji Kim, Won-Baek Kim, Kyoung Yoon Koo, Bo Ram Kim, Doohyun Kim, Seoyoun Lee, Hong Joo Son, Dae Youn Hwang, Dong Seob Kim, Chung Yeoul Lee, Heeseob Lee|
|Place of duty||Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea|
|Title||Optimal Fermentation Conditions of Hyaluronidase Inhibition Activity on Asparagus cochinchinensis Merrill by Weissella cibaria|
J. Microbiol. Biotechnol.2017 ;
|Abstract||This study was conducted to evaluate the hyaluronidase (HAase) inhibition activity of
Asparagus cochinchinesis (AC) extracts following fermentation by Weissella cibaria through
response surface methodology. To optimize the HAase inhibition activity, a central composite
design was introduced based on four variables: the concentration of AC extract (X1: 1–5%),
amount of starter culture (X2: 1–5%), pH (X3: 4–8), and fermentation time (X4: 0–10 days). The
experimental data were fitted to quadratic regression equations, the accuracy of the equations
was analyzed by ANOVA, and the regression coefficients for the surface quadratic model of
HAase inhibition activity in the fermented AC extract were estimated by the F test and the
corresponding p values. The HAase inhibition activity indicated that fermentation time was
most significant among the parameters within the conditions tested. To validate the model,
two different conditions among those generated by the Design Expert program were selected.
Under both conditions, predicted and experimental data agreed well. Moreover, the content of
protodioscin (a well-known compound related to anti-inflammation activity) was elevated
after fermentation of the AC extract at the optimized fermentation condition.|
|Key_word||Asparagus cochinchinensis Merrill, fermentation, HAase (HAase) inhibition activity, response surface methodology (RSM)|
Huang KC. 1993. The Pharmacology of Chinese Herbs. CRC Press, Boca Raton, FL, USA.
Lee DY, Choo BK, Yoon TS, Cheon MS, Lee HW, Lee AY, Kim HK. 2009. Anti-inflammatory effects of Asparagus cochinchinensis extract in acute and chronic cutaneous inflammation. J. Ethnopharmacol. 121: 28-34.
Xiong D, Yu LX, Yan X, Guo C, Xiong Y. 2011. Effects of root and stem extracts of Asparagus cochinchinensis on biochemical indicators related to aging in the brain and liver of mice. Am. J. Chin. Med. 39: 719-726.
Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP. 2015. Methyl protodioscin from the roots of Asparagus cochinchinensis attenuates airway inflammation by inhibiting cytokine production. Evid. Based Complement. Alternat. Med. 2015: 640846.
Samad NB, Debnath T, Hasnat A, Pervin M, Km DH, Jo JE, et al. 2014. Phenolic contents, antioxidant and anti-inflammatory activities of Asparagus cochinchinensis Merrill. J. Food Biochem. 38: 83-91.
Choo DY, Choo BK, Yoon TS, Cheon MS, Lee HW, Lee AY, Kim HK. 2009. Anti-inflammatory effects of Asparagus cochinchinensis extract in acute and chronic cutaneous inflammation. J. Ethnopharmacol. 121: 28-34.
Shen Y, Xu CL, Xuan WD, Li HL, Liu RH, Xu XK, Chen HS. 2011. A new furostanol saponin from Asparagus cohinchinensis. Arch. Pharm Res. 34: 1587-1591.
Zhang HJ, Sydara K, Tan GT, Ma C, Southavong B, Soejarto DD, et al. 2004. Bioactive constituents from Asparagus cochinchinensis. J. Nat. Prod. 67: 194-200.
Jung HG, Lee CW, Lee JH, Kim SJ, Kwon SJ, Kwon YS, et al. 2016. The new phytoformula containing Morus alba, Schizandra sinensis and Asparagus cochinchinensis inhibits lung inflammation in vitro and in vivo. Nat. Prod. Sci. 22: 70-75.
Lee HJ, Park JS, Yoon YP, Shin YJ, Lee SK, Kim YS, et al. 2015. Dioscin and methylprotodioscin isolated from the root of Asparagus cochinchinensis suppressed the gene expression and production of airway MUC5AC mucin induced by phorbol ester and growth factor. Phytomedicine 22: 568-572.
Wang M, Tadmor Y, Wu QL, Chin CK, Garrison SA, Simon JE. 2003. Quantification of protodioscin and rutin in asparagus shoots by LC/MS and HPLC methods. J. Agric. Food Chem. 51: 6132-6136.
Jang HJ, Kang MS, Yi SH, Hong JY, Hong SP. 2016. Comparative study on the characteristics of Weissella cibaria CMU and probiotic strains for oral care. Molecules 21: 1752.
Kang MS, Na HS, Oh JS. 2005. Coaggregation ability of Weissella cibaria isolates with Fusobacterium nucleatum and their adhesiveness to epithelial cells. FEMS Microbiol. Lett. 253: 323-329.
Lee KW, Park JY, Chun JY, Han NS, Kim JH. 2010. Importance of Weissella species during kimchi fermentation and future works. Korean J. Microbiol. Biotechnol. 38: 341-348.
Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, et al. 2015. The genus Weissella: taxonomy, ecology and biotechnological potential. Front. Microbiol. 6: 155.
Cameron E, Pauling L, Leibovitz B. 1979. Ascorbic acid and cancer: a review. Cancer Res. 39: 663-681.
Girish KS, Kemparaju K. 2007. The magic glue hyaluronan and its eraser hyaluronidase: a biological overview. Life Sci. 80: 1921-1943.
Guo X, Liu F, Zhu X, Su Y, Ling P. 2009. Expression of a novel hyaluronidase from Streptococcus zooepidemicus in Escherichia coli and its application for the preparation of HA oligosaccharides. Carbohydr. Polym. 77: 254-260.
Kakegawa H, Matsumoto H, Satoh T. 1999. Inhibitory effects of some natural products on the activation of hyaluronidase and their antiallergic action. Chem. Pharm. Bull. 40: 1439-1442.
Meyer K. 1947. The biological significance of hyaluronic acid hyaluronidase. Physiol. Rev. 27: 335-359.
Vincent JC, Lenormand H. 2009. How hyaluronan-protein complexes modulate the hyaluronidase activity: the model. Biophys. Chem. 145: 126-134.
Elson LA, Morgan WTJ. 1933. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem. J. 27: 1824-1828.
Kaegawa H, Matsumoto H, Endo K, Satoh T, Nonaka GI, Noshioka I. 1985. Inhibitory effects of tannins on hyaluronidase activation and on the degranulation from rat mesentery mast cells. Chem. Pharm. Bull. 33: 5079-5082.
Lee KK, Kim JH, Cho JJ, Choi JD. 1999. Inhibitory effects of 150 plant extracts on elastase activity, and their antiinflammatory effects. Int. J. Cosmet. Sci. 21: 71-82.
Haaland PD, Lust JC, Liddle FR, Curry JW. 1990. Dexter: a guide to selecting the best design for an industrial screening experiment. Ann. Math. Artif. Intell. 2: 179-196.
Liu JZ, Weng LP, Zhang QL. 2003. Optimization of glucose oxidase production by Aspergillus niger in benchtop bioreactor using response surface methodology. World J. Microbiol. Biotechnol. 19: 317-323.
Zhang ZF, Lv GY, Jiang X, Cheng JH, Fan LF. 2015. Extraction optimization and biological properties of a polysaccharide isolated from Gleoestereum incarnatum. Carbohydr. Polym. 117:185-191.
Zhang YJ, Li Q, Zhang YX, Wang D, Xing JM. 2012. Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM). J. Zhejiang Univ. Sci. B Biomed. Biotechnol. 13: 103-110.
Yoon CH, Bok HS, Choi DK, Row KH. 2012. Optimization condition of astaxanthin extract from shrimp waste using response surface methodology. Korean Chem. Eng. Res. 50:545-550.
Ghosh D, Hallenbeck PC. 2010. Response surface methodology for process parameter optimization of hydrogen yield by the metabolically engineered strain Escherichia coli DJT135. Bioresour. Technol. 101: 1820-1825.
Zhu T, Heo HJ, Row KH. 2010. Optimization of crude polysaccharides extraction from Hizikia fusiformis using response surface methodology. Carbohydr. Polym. 82: 106-110.