2017 ; Vol.27-4: 808~815
|Author||Eun-Hee Lee, Kyung-Suk Cho, Ahjeong Son|
|Place of duty||Department of Environmental Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea|
|Title||Detection and Quantification of Toxin-Producing Microcystis aeruginosa Strain in Water by NanoGene Assay|
J. Microbiol. Biotechnol.2017 ;
|Abstract||We demonstrated the quantitative detection of a toxin-producing Microcystis aeruginosa
(M. aeruginosa) strain with the laboratory protocol of the NanoGene assay. The NanoGene
assay was selected because its laboratory protocol is in the process of being transplanted into a
portable system. The mcyD gene of M. aeruginosa was targeted and, as expected, its
corresponding fluorescence signal was linearly proportional to the mcyD gene copy number.
The sensitivity of the NanoGene assay for this purpose was validated using both dsDNA mcyD
gene amplicons and genomic DNAs (gDNA). The limit of detection was determined to be 38
mcyD gene copies per reaction and 9 algal cells/ml water. The specificity of the assay was also
demonstrated by the addition of gDNA extracted from environmental algae into the
hybridization reaction. Detection of M. aeruginosa was performed in the environmental
samples with environmentally relevant sensitivity (~105 algal cells/ml) and specificity. As
expected, M. aeruginosa were not detected in nonspecific environmental algal gDNA over the
range of 2 × 100 to 2 × 107 algal cells/ml.|
|Key_word||Algal bloom, cyanobacteria, Microcystis aeruginosa, microcystin, NanoGene assay|
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