2017 ; Vol.27-3: 552~560
|Author||Sang Hyun Lee, Seonki Lee, Kibaek Lee, Chang Hyun Nahm, Sung-Jun Jo, Jaewoo Lee, Kwang-Ho Choo, Jung-Kee Lee, Chung-Hak Lee, Pyung-Kyu Park|
|Place of duty||School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea|
|Title||Enhancing the Physical Properties and Lifespan of Bacterial Quorum Quenching Media through Combination of Ionic Cross-Linking and Dehydration|
J. Microbiol. Biotechnol.2017 ;
|Abstract||Quorum quenching (QQ) bacteria entrapped in a polymeric composite hydrogel (QQ medium)
have been successfully applied in membrane bioreactors (MBRs) for effective biofouling
control. However, in order to bring QQ technology closer to practice, the physical strength
and lifetime of QQ media should be improved. In this study, enforcement of physical strength,
as well as an extension of the lifetime of a previously reported QQ bacteria entrapping hollow
cylinder (QQ-HC), was sought by adding a dehydration procedure following the cross-linking
of the polymeric hydrogel by inorganic compounds like Ca2+ and boric acid. Such prepared
medium demonstrated enhanced physical strength possibly through an increased degree of
physical cross-linking. As a result, a longer lifetime of QQ-HCs was confirmed, which led to
improved biofouling mitigation performance of QQ-HC in an MBR. Furthermore, QQ-HCs
stored under dehydrated condition showed higher QQ activity when the storage time lasted
more than 90 days owing to enhanced cell viability. In addition, the dormant QQ activity after
the dehydration step could be easily restored through reactivation with real wastewater, and
the reduced weight of the dehydrated media is expected to make handling and transportation
of QQ media highly convenient and economical in practice.|
|Key_word||PVA, media, cell immobilization, quorum quenching, membrane bioreactors|
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