2017 ; Vol.27-3: 616~623
|Author||Tran Xuan Ngoc Huy, Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, WonGi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim|
|Place of duty||Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea|
|Title||Intracellular Trafficking Modulation by Ginsenoside Rg3 Inhibits Brucella abortus Uptake and Intracellular Survival within RAW 264.7 Cells|
J. Microbiol. Biotechnol.2017 ;
|Abstract||Ginsenoside Rg3, a saponin extracted from ginseng, has various pharmacological and
biological activities; however, its effects against Brucella infection are still unclear. Herein, the
inhibitory effects of ginsenoside Rg3 against intracellular parasitic Brucella infection were
evaluated through bacterial infection, adherence assays, and LAMP-1 colocalization, as well as
immunoblotting and FACS for detecting MAPK signaling proteins and F-actin polymerization,
respectively. The internalization, intracellular growth, and adherence of Brucella abortus in
Rg3-treated RAW 264.7 cells were significantly decreased compared with the Rg3-untreated
control. Furthermore, an apparent reduction of F-actin content and intensity of F-actin
fluorescence in Rg3-treated cells was observed compared with B. abortus-infected cells without
treatment by flow cytometry analysis and confocal microscopy, respectively. In addition,
treating cells with Rg3 decreased the phosphorylation of MAPK signaling proteins such as
ERK 1/2 and p38 compared with untreated cells. Moreover, the colocalization of B. abortuscontaining
phagosomes with LAMP-1 was markedly increased in Rg3-treated cells. These
findings suggest that ginsenoside Rg3 inhibits B. abortus infection in mammalian cells and can
be used as an alternative approach in the treatment of brucellosis.|
|Key_word||B. abortus, Ginsenoside Rg3, intracellular growth, macrophage, inhibitory effect|
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