Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2017 ; Vol.27-1: 43~48

AuthorJoon Ha Lee, In-Woo Kim, Mi-Ae Kim, Mi-Young Ahn, Eun-Young Yun, Jae Sam Hwang
Place of dutyDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea
TitleAntimicrobial Activity of the Scolopendrasin V Peptide Identified from the Centipede Scolopendra subspinipes mutilans
PublicationInfo J. Microbiol. Biotechnol.2017 ; Vol.27-1
AbstractIn a previous study, we analyzed the transcriptome of Scolopendra subspinipes mutilans using next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V’s mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.
Full-Text
Key_wordAntimicrobial peptide, membrane damage, bacterial membrane, scolopendrasin V, Scolopendra subspinipes mutilans
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