Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2017 ; Vol.27-1: 36~42

AuthorViviana P. Rubio, Alejandra Bravo, Jorge Olmos
Place of dutyUniversidad Autónoma de Baja California (UABC), Marine Science Faculty, Ensenada, BC, México
TitleIdentification of a Bacillus thuringiensis Surface Layer Protein with Cytotoxic Activity against MDA-MB-231 Breast Cancer Cells
PublicationInfo J. Microbiol. Biotechnol.2017 ; Vol.27-1
AbstractIn this work, we isolated a surface layer protein (SLP) from a Bacillus thuringiensis (Bt) strain to evaluate it cytotoxic effects against MDA-MB-231 human breast cancer cells. AP11 was selected from a g roup o f Bt s trains u sing SLP o lig onucleotides developed from Bacillus conserved regions. The AP11 strain was grown in Luria Bertani medium until the late exponential phase; an 86 kDa protein was extracted using 5 M LiCl and identified by liquid chromatography– tandem mass spectrometry. It corresponded to a multispecies SLP highly similar to previously described SLPs in Bt. The MDA-MB-231 breast cancer cells LC50 was obtained using 0.25 μg/ml of the isolated SLP. HaCat non-cancerous cells presented 90% survival using the same protein concentration. Our data suggest that SLP cytotoxicity against MDA-MB-231 could be induced by an interaction with the CDH11 cell membrane receptor.
Full-Text
Key_wordBacillus thuringiensis, surface layer protein, breast cancer, cytotoxicity, anticancer, MDA-MB-231
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