2016 ; Vol.26-11: 1891~1907
|Author||Abdelrahman Saleh Zaky, Darren Greetham, Edward J. Louis, Greg A. Tucker, Chenyu Du|
|Place of duty||Division of Food Sciences, School of Biosciences, University of Nottingham, Nottingham LE12 5RD, UK,School of Applied Sciences, University of Huddersfield, Huddersfield HD1 3DH, UK,Department of Microbiology, Faculty of Agriculture, Cairo University, Giza 12613, Egypt|
|Title||A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology|
J. Microbiol. Biotechnol.2016 ;
|Abstract||Yeasts that are present in marine environments have evolved to survive hostile environments
that are characterized by high exogenous salt content, high concentrations of inhibitory
compounds, and low soluble carbon and nitrogen levels. Therefore, yeasts isolated from
marine environments could have interesting characteristics for industrial applications.
However, the application of marine yeast in research or industry is currently very limited
owing to the lack of a suitable isolation method. Current methods for isolation suffer from
fungal interference and/or low number of yeast isolates. In this paper, an efficient and nonlaborious
isolation method has been developed and successfully isolated large numbers of
yeasts without bacterial or fungal growth. The new method includes a three-cycle enrichment
step followed by an isolation step and a confirmation step. Using this method, 116 marine
yeast strains were isolated from 14 marine samples collected in the UK, Egypt, and the USA.
These strains were further evaluated for the utilization of fermentable sugars (glucose, xylose,
mannitol, and galactose) using a phenotypic microarray assay. Seventeen strains with higher
sugar utilization capacity than the reference terrestrial yeast Saccharomyces cerevisiae NCYC
2592 were selected for identification by sequencing of the ITS and D1/D2 domains. These
strains belonged to six species: S. cerevisiae, Candida tropicalis, Candida viswanathii, Wickerhamomyces
anomalus, Candida glabrata, and Pichia kudriavzevii. The ability of these strains for improved
sugar utilization using seawater-based media was confirmed and, therefore, they could
potentially be utilized in fermentations using marine biomass in seawater media, particularly
for the production of bioethanol and other biochemical products.|
|Key_word||Marine Yeast, Phenotypic microarray, Identification, Screening, Fermentation, Seawater|
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