Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2016 ; Vol.26-8: 1439~1445

AuthorSu-Kyoung Shin, Eunji Kim, Sungmi Choi, Hana Yi
Place of dutyBK21PLUS Program in Embodiment: Health-Society Interaction, Department of Public Health Sciences, Graduate School, Korea University, Seoul 02841, Republic of Korea
TitleCochleicola gelatinilyticus gen. nov., sp. nov., Isolated from a Marine Gastropod, Reichia luteostoma
PublicationInfo J. Microbiol. Biotechnol.2016 ; Vol.26-8
AbstractA yellow, rod-shaped, non-motile, gram-negative, and strictly aerobic bacterial strain, designated LPB0005T, was isolated from a marine gastropod, Reichia luteostoma. Here the genome sequence was determined, which comprised 3,395,737 bp with 2,962 protein-coding genes. The DNA G+C content was 36.3 mol%. The 16S rRNA gene sequence analysis indicated that the isolate represents a novel genus and species in the family Flavobacteriaceae, with relatively low sequence similarities to other closely related genera. The isolate showed chemotaxonomic properties within the range reported for the family Flavobacteriaceae, but possesses many physiological and biochemical characteristics that distinguished it from species in the closely related genera Ulvibacter, Jejudonia, and Aureitalea. Based on phylogenetic, phenotypic, and genomic analyses, strain LPB0005T represents a novel genus and species, for which the name Cochleicola gelatinilyticus gen. nov., sp. nov. is proposed. The type strain is LPB0005T (= KACC 18693T = JCM 31218T).
Supplemental Data
Key_wordCochleicola gelatinilyticus, Flavobacteriaceae, novel species, taxonomy
  1. Baek K, Jo H, Choi A, Kang I, Cho JC. 2014. Ulvibacter marinus sp. nov., isolated from coastal seawater. Int. J. Syst. Evol. Microbiol. 64: 2041-2046.
    Pubmed CrossRef
  2. Bernardet JF, Nakagawa Y, Holmes B. 2002. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int. J. Syst. Evol. Microbiol. 52: 1049-1070.
  3. Bowman JP. 2000. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int. J. Syst. Evol. Microbiol. 50: 1861-1868.
    Pubmed CrossRef
  4. Choi TH, Lee HK, Lee K, Cho JC. 2007. Ulvibacter antarcticus sp. nov., isolated from Antarctic coastal seawater. Int. J. Syst. Evol. Microbiol. 57: 2922-2925.
    Pubmed CrossRef
  5. Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW. 2007. EzTaxon: a Web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57: 2259-2261.
    Pubmed CrossRef
  6. da Costa MS, Albuquerque L, Nobre MF, Wait R. 2011. The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. Methods Microbiol. 38: 197-206.
  7. da Costa MS, Albuquerque L, Nobre MF, Wait R. 2011. The identification of polar lipids in prokaryotes. Methods Microbiol. 38: 165-181.
  8. Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791.
  9. Felsenstein J. 1993, posting date. PHYLIP (phylogenetic inference package) version 3.5c. Department of Genetics, University of Washington. [Online.]
  10. Jeon YS, Lee K, Park SC, Kim BS, Cho YJ, Ha SM, Chun J. 2014. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int. J. Syst. Evol. Microbiol. 64: 689-691.
    Pubmed CrossRef
  11. Jukes TH, Cantor CR. 1969. Evolution of protein molecules, pp. 21-132. In Munro HN (ed.). Mammalian Protein Metabolism. Academic Press, New York.
  12. Minnikin DE, Odonnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2: 233-241.
  13. Nedashkovskaya OI, Kim SB, Han SK, Rhee MS, Lysenko AM, Falsen E, et al. 2004. Ulvibacter litoralis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the green alga Ulva fenestrata. Int. J. Syst. Evol. Microbiol. 54: 119-123.
    Pubmed CrossRef
  14. Park S, Lee JS, Lee KC, Yoon JH. 2013. Jejudonia soesokkakensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Aureitalea Park et al. 2012. Antonie Van Leeuwenhoek 104: 139-147.
    Pubmed CrossRef
  15. Park S, Yoshizawa S, Inomata K, Kogure K, Yokota A. 2012. Aureitalea marina gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from seawater. Int. J. Syst. Evol. Microbiol. 62: 912-916.
    Pubmed CrossRef
  16. Powers EM. 1995. Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of foodborne and waterborne bacteria and yeasts. Appl. Environ. Microbiol. 61: 3756-3758.
    Pubmed Pubmed Central
  17. Reichenbach H. 1989. Order 1. Cytophagales Leadbetter 1974, 99AL, pp. 2011-2013. In Staley JT, Bryant MP, Pfenning N, Holt JG (eds.). Bergey's Manual of Systematic Bacteriology. The Williams & Wilkins Co., Baltimore.
  18. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, et al. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61: 539-542.
    Pubmed CrossRef Pubmed Central
  19. Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
  20. Sohpal VK, Dey A, Singh A. 2010. MEGA biocentric software for sequence and phylogenetic analysis: a review. Int. J. Bioinform. Res. Appl. 6: 230-240.
    Pubmed CrossRef
  21. Stackebrandt E, Goebel BM. 1994. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44: 846-849.
  22. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Ciufo S, Li WJ. 2013. Prokaryotic genome annotation pipeline. In Beck J, Benson D, Coleman J, Hoeppner M, Johnson M, Maglott D, et al. (eds.). The NCBI Handbook. National Center for Biotechnology Information, Bethesda, MD.
  23. Tindell BJ, Sikorski J, Smibert RA, Krieg NR. 2007. Phenotypic characterization and the principles of comparative systematics, pp. 330-393. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR (eds.). Methods for General and Molercular Microbiology. American Soceity for Microbiology, Washington, DC.
  24. Weeks OB. 1981. Preliminary studies of the pigments of Flavobacterium breve NCTC 11099 and Flavobacterium odoratum NCTC 11036, pp. 108-114. In Reichenbach H, Weeks OB (eds.). The Flavobacterium-Cytophaga Group. Gesellschaft fur Biotechnologische Forschung, Weinheim.

Copyright © 2009 by the Korean Society for Microbiology and Biotechnology.
All right reserved. Mail to
Online ISSN: 1738-8872    Print ISSN: 1017-7825    Powered by, Ltd