2016 ; Vol.26-9: 1593~1604
|Author||Sung Jun Jo, Hyeokpil Kwon, So-Yeon Jeong, Sang Hyun Lee, Hyun-Suk Oh, Taewoo Yi, Chung-Hak Lee, Tae Gwan Kim|
|Place of duty||School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea|
|Title||Effects of Quorum Quenching on the Microbial Community of Biofilm in an Anoxic/Oxic MBR for Wastewater Treatment|
J. Microbiol. Biotechnol.2016 ;
|Abstract||Recently, bacterial quorum quenching (QQ) has been proven to have potential as an
innovative approach for biofouling control in membrane bioreactors (MBRs) for advanced
wastewater treatment. Although information regarding the microbial community is crucial for
the development of QQ strategies, little information exists on the microbial ecology in QQMBRs.
In this study, the microbial communities of biofilm were investigated in relation to the
effect of QQ on anoxic/oxic MBRs. Two laboratory-scale MBRs were operated with and
without QQ-beads (QQ-bacteria entrapped in beads). The transmembrane pressure increase in
the QQ-MBRs was delayed by approximately 100-110% compared with conventional- and
vacant-MBRs (beads without QQ-bacteria) at 45 kPa. In terms of the microbial community, QQ
gradually favored the development of a diverse and even community. QQ had an effect on
both the bacterial composition and change rate of the bacterial composition. Proteobacteria
and Bacteroidetes were the most dominant phyla in the biofilm, and the average relative
composition of Proteobacteria was low in the QQ-MBR. Thiothrix sp. was the dominant
bacterium in the biofilm. The relative composition of Thiothrix sp. was low in the QQ-MBR.
These findings provide useful information that can inform the development of a new QQ
|Key_word||Membrane bioreactor, Biofouling, Biofilm, Quorum sensing, Quorum quenching, Microbial community|
American Public Health Association, the American Water Works Association, and the Water Environment Federation. 2005. Standard Methods for the Examination of Water and Wastewater. APHA/AWWA/WEF, Washington, DC.
Bell T, Newman JA, Silverman BW, Turner SL, Lilley AK. 2005. The contribution of species richness and composition to bacterial services. Nature 436: 1157-1160.
Brigmon R, Martin H, Aldrich H. 1997. Biofouling of groundwater systems by Thiothrix spp. Curr. Microbiol. 35:169-174.
Case RJ, Labbate M, Kjelleberg S. 2008. AHL-driven quorumsensing circuits: their frequency and function among the Proteobacteria. ISME J. 2: 345.
Cheong W-S, Kim S-R, Oh H-S, Lee SH, Yeon K-M, Lee C-H, Lee J-K. 2014. Design of quorum quenching microbial vessel to enhance cell viability for biofouling control in membrane bioreactor. J. Microbiol. Biotechnol. 24: 97-105.
Cheong W-S, Lee C-H, Moon Y-H, Oh H-S, Kim S-R, Lee SH, et al. 2013. Isolation and identification of indigenous quorum quenching bacteria, Pseudomonas sp. 1A1, for biofouling control in MBR. Ind. Eng. Chem. Res. 52: 1055410560.
Diggle SP, Griffin AS, Campbell GS, West SA. 2007. Cooperation and conflict in quorum-sensing bacterial populations. Nature 450: 411-414.
Drews A. 2010. Membrane fouling in membrane bioreactors characterisation, contradictions, cause and cures. J. Membr. Sci. 363: 1-28.
Fekete A, Kuttler C, Rothballer M, Hense BA, Fischer D, Buddrus-Schiemann K, et al. 2010. Dynamic regulation of Nacylhomoserine lactone production and degradation in Pseudomonas putida IsoF. FEMS Microbiol. Ecol. 72: 22-34.
Fuqua C, Winans SC. 1996. Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J. Bacteriol. 178: 435-440.
Gao D-W, Wen Z-D, Li B, Liang H. 2014. Microbial community structure characteristics associated membrane fouling in A/O-MBR system. Bioresour. Technol. 154: 87-93.
Hai FI, Yamamoto K, Lee C-H. 2013. Membrane Biological Reactors: Theory, Modeling, Design, Management and Applications to Wastewater Reuse. IWA Publishing, London, UK.
Hiraishi A, Muramatsu K, Urata K. 1995. Characterization of new denitrifying Rhodobacter strains isolated from photosynthetic sludge for wastewater treatment. J. Ferment. Bioeng. 79: 39-44.
Hu M, Wang XH, Wen XH, Xia Y. 2012. Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis. Bioresour. Technol. 117: 72-79.
Jahangir D, Oh H-S, Kim S-R, Park P-K, Lee C-H, Lee J-K. 2012. Specific location of encapsulated quorum quenching bacteria for biofouling control in an external submerged membrane bioreactor. J. Membr. Sci. 411: 130-136.
Kim H-W, Oh H-S, Kim S-R, Lee K-B, Yeon K-M, Lee C-H, et al. 2013. Microbial population dynamics and proteomics in membrane bioreactors with enzymatic quorum quenching. Appl. Microbiol. Biotechnol. 97: 4665-4675.
Kim J-H, Choi D-C, Yeon K-M, Kim S-R, Lee C-H. 2011. Enzyme-immobilized nanofiltration membrane to mitigate biofouling based on quorum quenching. Environ. Sci. Technol. 45: 1601-1607.
Kim S-R, Lee K-B, Kim J-E, Won Y-J, Yeon K-M, Lee C-H, Lim D-J. 2015. Macroencapsulation of quorum quenching bacteria by polymeric membrane layer and its application to MBR for biofouling control. J. Membr. Sci. 473: 109-117.
Kim S-R, Oh H-S, Jo S-J, Yeon K-M, Lee C-H, Lim D-J, et al. 2013. Biofouling control with bead-entrapped quorum quenching bacteria in membrane bioreactors: physical and biological effects. Environ. Sci. Technol. 47: 836-842.
Kim TG, Yun J, Cho K-S. 2015. The close relation between Lactococcus and Methanosaeta is a keystone for stable methane production from molasses wastewater in a UASB reactor. Appl. Microbiol. Biotechnol. 99: 8271-8283.
Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M, Glöckner FO. 2012. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and nextgeneration sequencing-based diversity studies. Nucleic Acids Res. 41: e1.
Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. 2013. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl. Environ. Microbiol. 79: 5112-5120.
Lee S, Park S-K, Kwon H, Lee SH, Lee K, Nam CH, et al. 2016. Crossing the border between laboratory and field:bacterial quorum quenching for anti-biofouling strategy in an MBR. Environ. Sci. Technol. 50: 1788-1795.
Lim S, Kim S, Yeon K-M, Sang B-I, Chun J, Lee C-H. 2012. Correlation between microbial community structure and biofouling in a laboratory scale membrane bioreactor with synthetic wastewater. Desalination 287: 209-215.
Meng F, Chae S-R, Drews A, Kraume M, Shin H-S, Yang F. 2009. Recent advances in membrane bioreactors (MBRs):membrane fouling and membrane material. Water Res. 43:1489-1512.
Miura Y, Watanabe Y, Okabe S. 2007. Membrane biofouling in pilot-scale membrane bioreactors (MBRs) treating municipal wastewater: impact of biofilm formation. Environ. Sci. Technol. 41: 632-638.
Mulder J. 2012. Basic Principles of Membrane Technology. Springer Science & Business Media, Berlin.
Oh H-S, Kim S-R, Cheong W-S, Lee C-H, Lee J-K. 2013. Biofouling inhibition in MBR by Rhodococcus sp. BH4 isolated from real MBR plant. Appl. Microbiol. Biotechnol. 97:10223-10231.
Oh H-S, Yeon K-M, Yang C-S, Kim S-R, Lee C-H, Park SY, et al. 2012. Control of membrane biofouling in MBR for wastewater treatment by quorum quenching bacteria encapsulated in microporous membrane. Environ. Sci. Technol. 46: 4877-4884.
Otawa K, Asano R, Ohba Y, Sasaki T, Kawamura E, Koyama F, et al. 2006. Molecular analysis of ammonia-oxidizing bacteria community in intermittent aeration sequencing batch reactors used for animal wastewater treatment. Environ. Microbiol. 8: 1985-1996.
Piasecka A, Souffreau C, Vandepitte K, Vanysacker L, Bilad RM, De Bie T, et al. 2012. Analysis of the microbial community structure in a membrane bioreactor during initial stages of filtration. Biofouling 28: 225-238.
Puskas A, Greenberg E, Kaplan S, Schaefer A. 1997. A quorum-sensing system in the free-living photosynthetic bacterium Rhodobacter sphaeroides. J. Bacteriol. 179: 7530-7537.
Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, et al. 2009. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol. 75: 7537-7541.
Spoering AL, Gilmore MS. 2006. Quorum sensing and DNA release in bacterial biofilms. Curr. Opin. Microbiol. 9: 133-137.
Venturi V. 2006. Regulation of quorum sensing in Pseudomonas. FEMS Microbiol. Rev. 30: 274-291.
Wang X, Hu M, Xia Y, Wen X, Ding K. 2012. Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in China. Appl. Environ. Microbiol. 78: 7042-7047.
Weerasekara NA, Choo K-H, Lee C-H. 2014. Hybridization of physical cleaning and quorum quenching to minimize membrane biofouling and energy consumption in a membrane bioreactor. Water Res. 67: 1-10.
Williams TM, Unz RF. 1985. Filamentous sulfur bacteria of activated sludge: characterization of Thiothrix, Beggiatoa, and Eikelboom type 021N strains. Appl. Environ. Microbiol. 49:887-898.
Wittebolle L, Marzorati M, Clement L, Balloi A, Daffonchio D, Heylen K, et al. 2009. Initial community evenness favours functionality under selective stress. Nature 458: 623-626.
Yeon K-M, Cheong W-S, Oh H-S, Lee W-N, Hwang B-K, Lee C-H, et al. 2008. Quorum sensing: a new biofouling control paradigm in a membrane bioreactor for advanced wastewater treatment. Environ. Sci. Technol. 43: 380-385.
Yeon K-M, Lee C-H, Kim J. 2009. Magnetic enzyme carrier for effective biofouling control in the membrane bioreactor based on enzymatic quorum quenching. Environ. Sci. Technol. 43: 7403-7409.
Zhao DY, Huang R, Zeng J, Yu ZB, Liu P, Cheng SP, Wu QL. 2014. Pyrosequencing analysis of bacterial community and assembly in activated sludge samples from different geographic regions in China. Appl. Microbiol. Biotechnol. 98:9119-9128.
Zhu P, Li M. 2012. Recent progresses on AI-2 bacterial quorum sensing inhibitors. Curr. Med. Chem. 19: 174-186.