2016 ; Vol.26-6: 1132~1139
|Author||Young Bin Im, Woo Bin Park, Myunghwan Jung, Suk Kim, Han Sang Yoo|
|Place of duty||Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea|
|Title||Evaluation of Th1/Th2-Related Immune Response against Recombinant Proteins of Brucella abortus Infection in Mice|
J. Microbiol. Biotechnol.2016 ;
|Abstract||Brucellosis is a zoonotic disease caused by Brucella, a genus of gram-negative bacteria.
Cytokines have key roles in the activation of innate and acquired immunities. Despite several
research attempts to reveal the immune responses, the mechanism of Brucella infection
remains unclear. Therefore, immune responses were analyzed in mice immunized with nine
recombinant proteins. Cytokine production profiles were analyzed in the RAW 264.7 cells and
naive splenocytes after stimulation with three recombinant proteins, metal-dependent
hydrolase (r0628), bacterioferritin (rBfr), and thiamine transporter substrate-binding protein
(rTbpA). Immune responses were analyzed by ELISA and ELISpot assay after immunization
with proteins in mice. The production levels of NO, TNF-α, and IL-6 were time-dependently
increased after having been stimulated with proteins in the RAW 264.7 cells. In naive
splenocytes, the production of IFN-γ and IL-2 was increased after stimulation with the
proteins. It was concluded that two recombinant proteins, r0628 and rTbpA, showed strong
immunogenicity that was induced with Th1-related cytokines IFN-γ, IL-2, and TNF-α more
than Th2-related cytokines IL-6, IL-4, and IL-5 in vitro. Conversely, a humoral immune
response was activated by increasing the number of antigen-secreting cells specifically.
Furthermore, these could be candidate diagnosis antigens for better understanding of
|Key_word||Brucella abortus, cytokine, immunogenicity, recombinant protein|
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