2016 ; Vol.26-3: 477~482
|Author||Nguyen Huu Hoang, Sung-Yong Hong, Nguyen Lan Huong, Je Won Park|
|Place of duty||Department of Biotechnology Convergent Pharmaceutical Engineering, SunMoon University, Asan 31460, Republic of Korea|
|Title||Biochemical Characterization of Recombinant UDP-Glucose:Sterol 3-O-Glycosyltransferase from Micromonospora rhodorangea ATCC 31603 and Enzymatic Biosynthesis of Sterol-3-O-β-Glucosides|
J. Microbiol. Biotechnol.2016 ;
|Abstract||A uridine diphosphate-glucose:sterol glycosyltransferase-encoding gene was isolated and
cloned from the established fosmid library of Micromonospora rhodorangea ATCC 27932 that
usually produces the aminoglycoside antibiotic geneticin. The gene consists of 1,185 base pairs
and encodes a 41.4 kDa protein, which was heterologously expressed in Escherichia coli
BL21(DE3). In silico analyses of the deduced gene product suggested that it is a member of the
family 1 glycosyltransferases. The recombinant protein MrSGT was able to catalyze the
transfer of a glucosyl moiety onto the C-3 hydroxy function in sterols (β-sitosterol,
campesterol, and cholesterol), resulting in the corresponding steryl glucosides (β-sitosterol-3-
O-β-D-glucoside, campesterol-3-O-β-D-glucoside, and cholesterol-3-O-β-D-glucoside). This
enzyme prefers phytosterols to cholesterol, and also shows substrate flexibility to some extent,
in that it could recognize a number of acceptor substrates.|
|Key_word||UDP-glucose sterol glycosyltransferase, Micromonospora rhodorangea, phytosterol-3-O-β-D-glucosides|
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